slightly enhanced the sensitivity of the PCR, but visual inspection of gel-resolved PCR products from feces was nearly equally sufficient (Table and Figure).
A total of 76 kids were generated, among them 5 kids tested positive by Southern blotting
Predicted restriction enzyme cut sites in the database sequences were compared with actual restriction mapping fragment sizes and with the data from Southern blotting
to determine the order of sequence contigs, An independent sequence assembly for this region obtained from Celera Genomics (GA_x6K02T2PU9B, as of 1 June 2002) was also used to fill in some gaps.
The Sequenom EpiTYPER system (which uses MALDI-TOF mass spectrometry for quantification) and Southern blotting
tools were used to determine the methylation output ratio and the activation ratio, respectively.
identified a plasmid of [approximately equal to] 150 kb harboring [bla.
The purified DNA can be used in a number of downstream applications including Real-Time PCR, Southern blotting
Step-by-step instructions are provided for staining microbes, viewing meiosis under the microscope, extracting chromatin, culturing peripheral blood lymphocytes, southern blotting
, and isolating chloroplasts from spinach leaves.
Although all steps prior to Southern blotting
had positive results, no bands were seen on the exposed film: therefore the presence and number of correctly targeted colonies could not be determined.
When the familiar broad smear of restricted DNA is visible after 50-100 volt-hours, full-scale electrophoresis for Southern blotting
In addition, the study revealed novel patterns of methylation mosaicism associated with female fragile X carriers that cannot be detected by Southern blotting
In our laboratory, Southern blotting
was conducted according to an established protocol (27) to confirm chimeric CYP21A1P/CYP21A2 genes.
After AluI digestion, the DNA was transferred to a nylon membrane for polymorphic guanine cytosine-rich repetitive sequence (PGRS) Southern blotting