Southern blotting


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A method developed by EM Southern for detecting and manipulating specific DNA sequences previously separated by gel electrophoresis using a radioactively- or chemically-labeled DNA probe with sequence similarity

Southern blotting

A method of identifying a fragment of DNA containing a specific sequence of bases. A mixture of fragments, produced by cutting DNA with restriction enzymes, is separated on a gel block by ELECTROPHORESIS. The fragments are denatured to single strand DNA and transferred, by blotting, to a nitrocellulose sheet. The position of the desired fragment can then be shown by hybridizing it with a DNA probe labelled with radioactive phosphorous that will cause a black line on an X-ray film. The method was named after its developer Edward M. Southern. A similar technique for RNA analysis has been humorously called ‘Northern blotting’ and the play on words has been extended to include ‘WESTERN BLOTTING’.

Southern transfer

or

Southern blotting

a method of transferring BANDS of DNA, separated by GEL ELECTROPHORESIS, to a membrane support. The technique is named after E. Southern, who devised it. Compare NORTHERN TRANSFER.
References in periodicals archive ?
For Southern blotting of T1 generation of transformants, 20 ug of DNA were digested with EcoRI to hybridization with probes.
Southern blotting slightly enhanced the sensitivity of the PCR, but visual inspection of gel-resolved PCR products from feces was nearly equally sufficient (Table and Figure).
1] DNA was screened by PCR and also by Southern blotting analysis.
All high molecular weight DNA extracts were susceptible to complete nuclease digestion (data not shown), as demonstrated for tobacco and soybean DNA in Southern blotting and detection systems.
restriction fragment length polymorphism (RFLPs), short tandem repeats (STS) or variable number tandem repeats (VNTRs) which can be identified by Southern blotting or the polymerase chain reaction (PCR).
Families of closely related genes are difficult to analyze using standard techniques--because of high sequence similarity, a closely related gene or pseudogene can easily be mistaken for the target gene during Southern blotting or PCR amplifications.
This, in turn, is only possible when knowledge of the southern blotting technique, of the overall procedure and of biology are actively integrated.
The most conserved region of our native FCP amino acid sequence was used to design a 100 bp probe for Southern blotting of E.
The resulting putative auxotrophs will be characterized, sequenced, and confirmed by Southern blotting.
SB column indicates TCR[Beta] gene rearrangement positivity as determined by Southern blotting using TCR[Beta] probe; TGGE, clonality as determined by polymerase chain reaction amplification of TCR[Gamma], region; CTCL, cutaneous T-cell lymphoma, including mycosis fungoides; PTCL, peripheral T-cell lymphoma; LGL, large granular cell leukemia; PLL, prolymphocytic leukemia; PTLD, posttransplant lymphoproliferative disorder; CLL/SLL, chronic lymphocytic leukemia/small lymphocytic lymphoma; FL, follicular lymphoma; MCL, mantle cell lymphoma; MALToma, mucosa-associated lymphoid tissue lymphoma; and LCL, large cell lymphoma.
Examples would be the Appleton layer, Bell's palsy, Brewster's law, Brucellosis, Brownian movement, degrees Kelvin, Dewar flask, Graham's law, Higgs particle, Kerr effect, Liston splint, McLaggan diesel engine, McLaurin's theorem, Newland's law, Rankine cycle, Southern blotting method, and the Stirling engine.
In situ hybridization, immunocytochemistry, confocal immunoflourescent microscopy, and Southern blotting demonstrated the presence of gamma tubulin, [alpha]-tubulin, EF1[alpha], and several RNAs homologous to Xenopus RNAs implicated in embryonic axis formation or germ cell determination.

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