Southern blot(redirected from Southern Blot Hybridisation)
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Related to Southern Blot Hybridisation: Southern blot analysis, Southern transfer, Southern blotting method
a technique for detecting specific DNA sequences following agar gel electrophoresis of a set of DNA restriction enzyme digestion fragments. The fragments after electrophoresis are transferred to a nitrocellulose or nylon membrane by applying the membrane to the gel; the membrane is probed with a radioprobe that hybridizes to one or more of the separated fragments and used to produce an autoradiogram. Named for Edward Southern.