and analysis in yeast transformants.
Double-strand breaks stimulate the cell's natural DNA-repair processes, namely homologous recombination and non-homologous end joining, to induce site-specific mutagenesis
Genoplasty is a unique site-specific mutagenesis
technology that allows precise changes to be inserted into target genes which may be used to introduce genetic improvements without the need for foreign DNA.
played an important role in the solution of the structure in that the cysteine mutants C865, C1 14S, and C173S were used to help locate the positions of heavy atoms and to align the sequence with the model derived from the experimental phases.
4]-methylthymine in vivo determined by an enzymatic approach to site-specific mutagenesis
Rapid and efficient site-specific mutagenesis
without phenotypic selection.
by a propanodeoxyguanosine adduct carried on an M13 genome.
His group has also probed the factors determining and controlling the catalytic activities and specificities of the enzymes of asymmetric synthetic value, using molecular graphics, molecular dynamics, and site-specific mutagenesis
For about a decade, researchers have used a technique called site-specific mutagenesis
to interchange amino acids at individual locations in a protein and study the functional consequences.