Sephadex


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Sephadex

/Seph·a·dex/ (sef´ah-deks) trademark for cross-linked dextran beads. Various forms are used in chromatography.
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The heterologously expressed rYML5 was purified by CM-Sepharose FF and Sephadex G-75 column (Figure3e).
The n-BuOH portion was separated by D101 macroporous resin, repeated silica gel and Sephadex LH-20 column chromatography (CC) and preparative TLC to afford violaic A (1), violaic B (2), violalide (3) and palmitic acid (13).
The SDS-PAGE result was comparable to that of nonreduced hCG[beta]cf purified with the Sephadex G-75 column, as previously reported (10).
5 M NaCl, and the other was Sephadex G-100 gel filtration.
The reaction was stopped with 1m L-lysine and excess glutaraldehyde removed by passing mixture through a Sephadex G25 column.
The samples were then purified on Sephadex columns and run on five percent Long Ranger polyacrylamide gels in an ABI 377XL automated sequencer (Applied Biosystems, Foster City, CA, USA).
The precipitate was collected and dried in vacuum, and then purified through a Sephadex C-25 column using the aqueous solution of [NH.
The PSAO containing mixture was loaded onto a column of Sephadex G-200 ([empty set] = 1.
0 was reduced by sodium dithionite, which was removed by gel filtration in a Sephadex G-25 column.