Scatchard plot | definition of Scatchard plot by Medical dictionary
Scat·chard plot (skatch'erd),
1. a graphic representation used in the analysis of binding phenomena in which the concentration of bound ligand divided by the concentration of free ligand is plotted against the concentration of bound ligand;
2. similar to (1), except the concentration of the bound ligand is on the vertical axis.
Scatchard, George, U.S. chemist and biochemist, 1892-1973.
Scatchard plot - a graphical representation used in the analysis of binding phenomena.
References in periodicals archive
The data were evaluated in the form of a Scatchard plot, to determine the affinity and binding capacity of the imprinted polymer, expressed by affinity constants (K) and apparent maximum number ([B.
The Scatchard plot of the batch-binding experiments shows that in the imprinted S-MIPs there are two classes of binding sites (visible as two tangents).
The resulting Scatchard plot
indicated that albumin had 1 high-affinity site and perhaps 2 or more weaker sites (2).
analysis  of the purified antiserum exposed a homogeneous antibody population.
But although Scatchard plots
show that cadmium's affinity for the receptor is quite high (dissociation constant is [10.
5 [micro]M were indicated by biphasic Scatchard plots
Figure 4b shows that the Scatchard plots
were only a single straight line, which illustrated that there was only a class of binding sites populated in the nonimprinted microspheres.
were fitted by linear regression analysis and compared using analysis of covariance (ANCOVA).
Individual values of affinity and the coefficient of correlation for linearity of the Scatchard plots
are shown in Table 1.