Scatchard plot

Scat·chard plot

1. a graphic representation used in the analysis of binding phenomena in which the concentration of bound ligand divided by the concentration of free ligand is plotted against the concentration of bound ligand;
2. similar to (1), except the concentration of the bound ligand is on the vertical axis.
[George Scatchard]


George, U.S. chemist and biochemist, 1892-1973.
Scatchard plot - a graphical representation used in the analysis of binding phenomena.
References in periodicals archive ?
The data were evaluated in the form of a Scatchard plot, to determine the affinity and binding capacity of the imprinted polymer, expressed by affinity constants (K) and apparent maximum number ([B.
The Scatchard plot of the batch-binding experiments shows that in the imprinted S-MIPs there are two classes of binding sites (visible as two tangents).
Rosenthal transformations of the data are presented as Scatchard plot for illustration (Figure 1B).
The resulting Scatchard plot indicated that albumin had 1 high-affinity site and perhaps 2 or more weaker sites (2).
The unchanged structure-activity relations of peptide binding suggest that the detergent did not adversely affect the ligand binding site, but the curvilinear Scatchard plot does indicate some heterogeneity.
Scatchard plot analysis [14] of the purified antiserum exposed a homogeneous antibody population.
9728), and the values of Kd and qmax, calculated from the slope and intercept of Scatchard plots, are 62.
But although Scatchard plots show that cadmium's affinity for the receptor is quite high (dissociation constant is [10.
Figure 4b shows that the Scatchard plots were only a single straight line, which illustrated that there was only a class of binding sites populated in the nonimprinted microspheres.
Individual values of affinity and the coefficient of correlation for linearity of the Scatchard plots are shown in Table 1.