Tenders invited for Supply of nutrient agar, macconkey agar, muller hinton agar, sabouraud dextrose agar
and selenite broth etc.
It was inoculated onto blood agar (BioMerieux[R]), chocolate agar (BioMerieux[R]), and Sabouraud dextrose agar
(Britania[R]) and incubated at 28 and 37 [degrees]C.
One ml from each (prepared sputum, bronchoalveolar lavage, plural fluid) specimens were transferred aseptically into three sterilized nine cm Petri dishes and 15-20 ml of pre-solidifying Sabouraud dextrose agar
medium  were added to each plate.
The Sabouraud Dextrose Agar
(SDA) was prepared in screw cap tubes and autoclaved.
Pus samples were inoculated on blood agar, chocolate agar, Sabouraud Dextrose agar
and Maconkey agar plates and incubated at 37degC overnight.
Specimens of skin scraping were taken from each individual and were inoculated on Sabouraud dextrose agar
for seven days.
Fungal culture on Sabouraud dextrose agar
showed growth of green, velvety, and powdery colonies highly suggestive of Aspergillus fumigatus .
Out of 18 (18%) cases where fungal elements could not be identified by direct microscopic examination, 9 cases were positive for growth on Sabouraud dextrose agar
medium and remaining 9 cases did not show any growth on this medium.
In Sabouraud dextrose agar
, bronchoscopic brush specimen culture grew white and woolly colonies of fungus, and it displayed as annular area in the colonies central [Figure 1]c, [Figure 1]d, [Figure 1]e.
Firstly, sabouraud dextrose agar
and sabouraud dextrose broth cultivation environment was permissibly and standardly prepared; sabouraud dextrose agar
and sabouraud dextrose broth was purchased from Germany's MERCK Inc.
The swab samples were inoculated on Sabouraud Dextrose Agar
(SDA) and Dermatophyte Test Medium (DTM) to determine presence of any fungus.
The swabs were streaked for isolation onto Columbia agar supplemented with 5% sheep blood, MacConkey agar and Sabouraud dextrose agar