In this study, the effects of aberrant methylation of CpG islands within the promoter region of SOCS1 and SOCS3 genes were demonstrated in the pathogenesis of PV, ET, PMF, and secondary erythrocytosis/thrombocythemia.
A cohort of 87 patients, which included 19 cases of PV, 21 cases of ET, 5 cases of PMF, and 42 cases of secondary erythrocytosis/thrombocythemia, was enrolled to investigate the role of SOCS1 and SOCS3 promoter methylation.
Methylation Analysis of SOCS1 and SOCS3 CpG Islands: Genomic DNA from patients and controls were modified with sodium bisulfite using the CpGenomeTM Fast.
4 pmol of each primer for SOCS1, and 1 pmol of each primer for SOCS3, using 1.
Chi-square (chi-square, Fisher exact, or likelihood ratio) tests were used for comparisons of JAK2 and SOCS3 among sub-groups.
Primary disorder Participants JAK2 SOCS1 SOCS3 (*) (n = 116) V617F Polycythemia 19 17 / 19 0 / 19 5 / 19 4/5 vera (89.
SOCS3 promoter methylation represents an important mechanism in the pathogenesis of MPNs.
We next compared the MPNs among themselves for the frequency of SOCS3 promoter methylation.
In this study, secondary erythrocytosis/thrombocythemia patients were also analyzed for SOCS3 promoter methylation.
The coincidental association of SOCS3 promoter methylation and JAK2 V617F mutation is an important finding.