SDS-PAGE

(redirected from SDS-polyacrylamide gel electrophoresis)
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SDS-PAGE

sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
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Positive cell lines synthesized a protein of approximately 26 kDa when analyzed by SDS-polyacrylamide gel electrophoresis, which corresponds to the predicted size of the bcl-2 protein.
SDS-polyacrylamide gel electrophoresis (reducing conditions) showed the LPS-elicited exudate to contain major protein bands at 126, 93, 83, 79, 48, 47, 26, 23, 17, 16, 15, and 6 kDa and a number of minor bands between 45 and 30 kDa.
1997) had used 2 dimensional SDS-polyacrylamide gel electrophoresis (2D SDS-PAGE) technique to identify potential bovine serum encephalitis-specific markers on bovine colony stimulating factor samples.
Clearly limulin and Limulus CRP are closely related: both proteins bind to PE-affinity resins; both have the same native ([similar to]300 kDa--by size exclusion chromatography on Sephacryl S-300 HR resin) and subunit [[similar to]24-28 kDa--by SDS-polyacrylamide gel electrophoresis (PAGE)] molecular masses; and both have the same amino terminal peptide sequence (LEEGEGITSKV), and a polyclonal antiserum produced against purified Limulus CRP cross-reacts with limulin.
The SDS-polyacrylamide gel electrophoresis was carried out by the method of Laemmli [29] under reduced and non-reduced conditions in 12% acrylamide mini-slab gel.