nuclease (redirected from S1 nuclease)

nuclease /nu·cle·ase/ (noo´kle-ās) any of a group of enzymes that split nucleic acids into nucleotides and other products.

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nu·cle·ase (nkl-s, -z)

n.

Any of several enzymes, such as endonuclease and exonuclease, that hydrolize nucleic acids.

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nuclease

any of a group of enzymes that cleave or digest nucleic acids into fragments or single nucleotides.

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S1 nuclease

a single-strand-specific endonuclease that degrades DNA and RNA to nucleoside 5′-monophosphates. The enzyme is a single polypeptide chain of molecular weight 32,000 and is isolated from 'takadiesterase', a digestive enzyme preparation from Aspergillus oryzae. Widely used in molecular biology to map the position of mRNA to its DNA, and also used to remove single-stranded tails from DNA fragments, to produce blunt ends to open up hairpin-loop structures.