For instance, long reads are critical for researchers trying to interpret genetic data from human leukocyte antigen (HLA) genes or 16S ribosomal genes
The Nest 1 reaction amplified approximately 794 to 825 bp of the small subunit of the Plasmodium ribosomal gene
(depending on the Plasmodium species; P.
For major ribosomal gene
mapping we used ITS1, a specific probe generated by PCR using the primers 1TS1CA-CB (ITS1CA: 5'-GTTTCCGTAGGTG AACCTG-3', ITS1CB: 5'-CTCGTCTGATCTGAGGTCG-3) (Heath et al.
Using the 16S ribosomal gene
, the researchers identified 35 bacterial species associated with the syndrome.
Once the HMA-generated partial sequence information was available, the investigators were able to complete ribosomal gene
sequencing work for these and related organisms, and to develop highly specific PCR assays for P.
Cloning of selected 16S ribosomal gene
fragments from crystalline styles (1,500 bp or 550 bp) was performed using the TOPO-cloning kit (Invitrogen, Darmstadt, Germany) following the manufacturer's protocol.
Further, the ribosomal gene
sequence data indicate that archeans are more closely related to eukaryotes than to bacteria.
They then collected bacteria from both the inside and outside of these sections and searched the organisms for a particular gene, known as the 16S ribosomal gene
The quantity and quality of the tcRNA were evaluated using an Agilent 2100 bioanalizer (Agilent Technologies, Wilmington, DE) and mRNA expression for Cx26, Cx32 and Cx43 was quantified and normalized to expression of the housekeeping 18s ribosomal gene
by real time RT-PCR using the ABI Prism 7000 sequence detection system (Applied Biosystems, Foster City, CA).
Differences in mitochondrial 16S ribosomal gene
sequences allow discrimination among American (Crassostrea virginica) and Asian (C.
Although the global prevalence of pneumococcal strains with macrolide resistance conferred by ribosomal gene
mutations remains low (<2%), a study of macrolide resistance mechanisms among S.
Mapping 18S ribosomal genes
in fish of the genus Brycon (Characidae) by fluorescent in situ hybridization (FISH).