Reverse transcription-polymerase chain reaction

Reverse transcription-polymerase chain reaction; RT-PCR

A method of polymerase-chain-reaction amplification of nucleic acid sequences that uses RNA as the template for transcribing the corresponding DNA using reverse transcriptase.
Mentioned in: Noroviruses
References in periodicals archive ?
Microscopy and Reverse Transcription-Polymerase Chain Reaction
Clinical significance of molecular detection of carcinoma cells in lymph nodes and peripheral blood by reverse transcription-polymerase chain reaction in patients with gastrointestinal or breast carcinomas.
Analysis of plasma viral RNA levels during acute dengue virus infection using quantitative competitor reverse transcription-polymerase chain reaction.
Reverse transcription-polymerase chain reaction (RT-PCR) was performed using a TITANIUM one-step RT-PCR kit (Clontech, San Jose, CA) and a GeneAmp PCR system 9700 (Applied Biosystems, Foster City, CA) according to the kit's instructions.
Using reverse transcription-polymerase chain reaction (RT-PCR) we determined that both subtypes are expressed in the brain, skin, kidney, gill, testis and ovary.
The HCV genotype is determined by means of a target specific amplification using reverse transcription-polymerase chain reaction (RT-PCR) to generate amplified product from the specific HCV RNA sequences.
Genus-specific detection of alphaviruses by a semi-nested reverse transcription-polymerase chain reaction.
In this study we used quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to investigate discrepant results between the National Institute of Environmental Health Sciences cDNA and Affymetrix oligo platforms used to evaluate hepatic gene expression changes in rats exposed to methapyrilene.
Reliability of reverse transcription-polymerase chain reaction (RT-PCR)-based assays for the detection of circulating tumour cells: a quality-assurance initiative of the EORTC Melanoma Cooperative Group.
Our laboratory is able to provide expertise in an efficient gene expression approach, kinetic Reverse Transcription-Polymerase Chain Reaction (kRT-PCR).
Serum from 2 Norwegians with tickborne encephalitis (TBE) (1 of whom was infected in Denmark) and 810 Norwegian ticks were tested for TBE virus (TBEV) RNA by reverse transcription-polymerase chain reaction.

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