Reverse transcription-polymerase chain reaction

Reverse transcription-polymerase chain reaction; RT-PCR

A method of polymerase-chain-reaction amplification of nucleic acid sequences that uses RNA as the template for transcribing the corresponding DNA using reverse transcriptase.
Mentioned in: Noroviruses
References in periodicals archive ?
Reverse transcription-polymerase chain reaction (RT-PCR) was conducted as described previously [2].
On January 15, 2016, MSPP reported that five specimens were positive for Zika virus RNA using the Trioplex reverse transcription-polymerase chain reaction (RT-PCR) assay (3).
Using chikungunya virus RNA samples, the RT-RPA assay detected down to 80 genome copies per reaction within 15 minutes, a time period four to six times faster than other molecular diagnostic techniques, such as reverse transcription-polymerase chain reaction (RT-PCR).
Given the new data, CDC issued interim guidance that Zika virus real-time reverse transcription-polymerase chain reaction testing should be performed on both urine and serum specimens collected within seven days of the onset of symptoms.
According to the Centers for Disease Control and Prevention (CDC), during the first week after onset of symptoms, Zika virus disease can often be diagnosed by performing real-time reverse transcription-polymerase chain reaction (RT-PCR) on serum.
Patients with suspected Zika virus infection who have negative real-time reverse transcription-polymerase chain reaction (rRT-PCR) test results are not automatically in the clear, officials at the Centers for Disease Control and Prevention warned in new guidelines on the interpretation of Zika virus antibody test results.
Currently, the College of American Pathologists/International Association for the Study of Lung Cancer/Association for Molecular Pathology (CAP/IASLC/AMP) guideline recommends using fluorescence in situ hybridization (FISH) as the gold standard for detecting ALK translocations; reverse transcription-polymerase chain reaction (RT-PCR) is not recommended because of concerns regarding a higher failure rate of an RNA-based assay in routine formalin-fixed, paraffin-embedded (FFPE) pathology material, as well as the risk of false negatives due to variability in the EML4-ALK fusion structure and the existence of other ALK fusion partners.
SAN FRANCISCO -- As the availability of more sensitive diagnostic methods such as reverse transcription-polymerase chain reaction testing become more widespread, noroviruses are increasingly being recognized as important enteric pathogens in diverse populations.
Clinical significance of molecular detection of carcinoma cells in lymph nodes and peripheral blood by reverse transcription-polymerase chain reaction in patients with gastrointestinal or breast carcinomas.
Analysis of plasma viral RNA levels during acute dengue virus infection using quantitative competitor reverse transcription-polymerase chain reaction.
Reverse transcription-polymerase chain reaction (RT-PCR) was performed using a TITANIUM one-step RT-PCR kit (Clontech, San Jose, CA) and a GeneAmp PCR system 9700 (Applied Biosystems, Foster City, CA) according to the kit's instructions.
Using reverse transcription-polymerase chain reaction (RT-PCR) we determined that both subtypes are expressed in the brain, skin, kidney, gill, testis and ovary.

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