reverse transcriptase polymerase chain reaction

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reverse transcriptase polymerase chain reaction (RT-PCR),

a process for specific mRNA amplification wherein reverse transcriptase added to the in vitro reaction uses mRNA as a template to produce one cDNA, which is then amplified by the usual PCR.

re·verse trans·crip·tase pol·y·mer·ase chain re·ac·tion

(RT-PCR) (rē-vĕrs' tran-skrip'tās pol-im'ĕr-ās chān rē-ak'shŭn)
A process for specific mRNA amplification wherein reverse transcriptase added to the in vitro reaction uses mRNA as a template to produce one cDNA, which is then amplified by the usual PCR.
References in periodicals archive ?
Xpert Xpress Flu utilises automated real-time reverse transcription polymerase chain reaction (RT-PCR) to detect Influenza A and B RNA.
The participants learned how to do just that using the reverse transcription polymerase chain reaction (RT-PCR) molecular detection technique at the IAEA laboratories in Seibersdorf, Austria.
Methods: Reverse transcription polymerase chain reaction and immunohistochemistry were used to determine the expression of Hsulf-1 in HCC.
Oropharyngeal and cloacal swabs tested positive for influenza A virus H5N8 by real-time reverse transcription polymerase chain reaction.
4) Subsequent serological and molecular work confirmed this finding in camels connected to human cases in Qatar, which were positive for MERS-CoV by real time reverse transcription polymerase chain reaction (rRT-PCR) from nasal swabs.
The test measures the body's genetic response to viral infection using a reverse transcription polymerase chain reaction (RT-PCR) TaqMan low-density array (TLDA) platform.
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.
The E gene was amplified by using reverse transcription polymerase chain reaction (RT-PCR) as described previously (5).
2000i Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.
Overall, reverse transcription polymerase chain reaction tests are approximately 70% sensitive in the first few days after onset of the infection.
Laboratory diagnostic techniques used included viral isolation, hemagglutinin inhibition serology, and multiplex, reverse transcription polymerase chain reaction.
Our examination of footplate fragments by reverse transcription polymerase chain reaction testing in 95 patients with otosclerosis revealed the presence of measles virus RNA in 83% of cases.

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