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reverse transcription

   Also found in: Dictionary/thesaurus, Acronyms, Encyclopedia, Wikipedia 0.01 sec.
reverse transcription
n.
The process by which DNA is synthesized from an RNA template.

transcription
the synthesis of an RNA copy from a nucleotide sequence in a limited region of DNA. See also deoxyribonucleic acid and transcriptase.

transcription-control region
the cis-acting DNA sequences regulating transcription of a specific gene.
transcription factor (TF)
a general term for any protein, other than RNA polymerase, required to initiate or regulate transcription in eukaryotic cells. General TFs are involved in the formation of the transcription-preinitiation complexes near the start site and are required for transcription of all genes; specific TFs stimulate (activators) or inhibit (suppressors) transcription of particular genes by binding to their regulatory sequences.
nested set transcription
a hallmark of the replication strategy of coronaviruses, toroviruses and arteriviruses, in which a nested set of subgenomic mRNAs are generated having identical 5′-leader sequences and a common 3′-termini, but because of discontinuous transcription each mRNA has a unique coding sequence and is transcribed into a unique viral protein.
reverse transcription
the synthesis of a DNA copy from a RNA template, catalyzed by reverse transcriptase.
transcription unit
see operon.


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Spleen and kidney homogenates were examined for pestivirus nucleic acid by reverse transcription PCR by using panpestivirus primers (Pesti 3 and Pesti D) (6,7).
To further explore the mechanism of sECC, hepatic 3-hydroxy-3-methyglutaryl-CoA reductase (HMG-CoA reductase), cholesterol 7 [alpha]-hydroxylase (CYP7A1), and low density lipoprotein receptor (LDL receptor) expressions in cholesterol homeostasis were analyzed by reverse transcription PCR.
The study data was quantified by reverse transcription polymerase chain reaction (PCR).
 
 
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