reverse transcriptase

(redirected from Reverse transcribed)
Also found in: Dictionary, Thesaurus, Encyclopedia.

reverse transcriptase

 [re-vers´ tran-skrip´tās]
an enzyme of RNA viruses that catalyzes the transcription of RNA to DNA, which is then incorporated into the genome of the host cell. This is the reverse of the usual mechanism for replication of genetic information; in the presence of this enzyme it is the RNA that serves as the template for DNA copies. It is one mechanism by which reproduction of cancer cells is facilitated. Called also RNA-directed DNA polymerase.

re·verse tran·scrip·tase

RNA-dependent DNA polymerase, present in virions of RNA tumor viruses (retroviruses).

reverse transcriptase

/re·verse tran·scrip·tase/ (re-vers´ tran-skrip´tās) an enzyme that catalyzes the template-directed, step-by-step addition of deoxyribonucleotides to the end of a DNA or RNA primer or growing DNA chain, using a single-stranded RNA template; it occurs in retroviruses and the DNA formed is an intermediate in the formation of progeny RNA.

reverse transcriptase

n.
A polymerase that catalyzes the formation of DNA on an RNA template, found in the retroviruses and in certain other viruses, such as hepatitis B virus.

reverse transcriptase (RT)

[revers′tran-skrip′tās]
an enzyme of RNA viruses that catalyzes the transcription of RNA to DNA, which is then incorporated into the genome of the host cell. This is the reverse of the usual mechanism for replication of genetic information; in the presence of this enzyme, it is the RNA that serves as the template for DNA copies. It is one mechanism by which reproduction of cancer cells is facilitated. Also called RNA-dependent DNA polymerase. See also retrovirus.

re·verse trans·crip·tase

(RT) (rē-vĕrs' tran-skrip'tās)
RNA-dependent DNA polymerase, present in virions of RNA tumor viruses.

reverse transcriptase

An enzyme that allows certain viruses, notable HIV, to synthesize double strand DNA from a single strand of RNA so that it can be incorporated into the genome of the host cell. Such viruses are called retroviruses.

reverse transcriptase

an RNA-dependent DNA polymerase that catalyses the synthesis of DNA from deoxyribonucleoside-5′-triphosphate, using RNA as a template.

re·verse trans·crip·tase

(rĕ-vĕrs' tran-skrip'tās)
RNA-dependent DNA polymerase, present in virions of RNA tumor viruses.

reverse transcriptase (transkrip´-tās),

n an enzyme within a retrovirus that converts its ribonucleic acid into deoxyribonucleic acid (DNA), which then penetrates the cell nucleus and joins the host's DNA.

reverse transcriptase

see reverse transcriptase.
References in periodicals archive ?
The mRNA component of the total RNA could be selectively reverse transcribed to the corresponding cDNA and amplified by PCR.
Total RNA (1 [micro]L) was reverse transcribed in a 20-[micro]L reaction mixture using murine leukemia virus reverse transcriptase (50 U) in 1 x PCR buffer, 5 mM Mg[Cl.
In a final volume of 20 [micro]L, 1 [micro]g of total RNA was reverse transcribed to cDNA using 200 U of Moloney murine leukemia virus reverse transcriptase (Invitrogen).
Ten microliters of RNA was reverse transcribed by using SuperScript III RNaseH (-) reverse transcriptase according to the manufacture's instructions (Invitrogen, Carlsbad, CA, USA).
Four dilutions of ABCA1 cRNA were reverse transcribed in parallel to the sample material to be analyzed, and 2 [micro]L of each reverse transcription reaction was amplified with the probes for ABCA1 and PBGD in the LightCycler.
Viral RNA is reverse transcribed with random hexamers, followed by real-time PCR.
5] and 170 PFU/mL), reverse transcribed to cDNA with random primers, and serially diluted (10-fold), before PCR amplification with both RdRp and Nspl4 primer sets.
RNA (1-5 [micro]g) was reverse transcribed into cDNA in 20 [micro]L of 1X transcription buffer containing 1.

Full browser ?