reverse genetics

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reverse genetics

a conceptual approach allowing the study of gene function; it can be summarized as "from gene to phenotype" as opposed to forward genetics, which can be summarized as "from phenotype to gene." It uses structural changes in the gene by deletion through gene knockout or disruption by site-directed mutagenesis, and then investigating the effects of such alterations on the phenotype of the cell or organism.
Synonym(s): positional cloning

reverse genetics

methods such as antisense nucleic acids and site-directed mutagenesis that are used to selectively study gene function. Contrasts with classical genetics which depends on the isolation and analysis of cells (animals) with random mutations that can be identified.
References in periodicals archive ?
Objective: ExpoSEED aims to integrate forward and reverse genetic approaches to dissect the molecular mechanisms that control seed/kernel (hereafter referred as seed) yield in model species and to transfer the acquired knowledge to selected crops as legumes and cereals.
Scientists from Europe and the US discuss coronavirus and rhabdovirus reverse genetics, reverse genetic tools to study the hepatitis C virus, calcivirus genetics, modification of the measles virus and application to pathogenesis studies, bunyavirus reverse genetics and applications to studying interactions with host cells, using reverse genetics to improve influenza vaccines, Bluetongue virus reverse genetics, genetic modification in mammalian orthoreoviruses, and reverse genetics and quasispecies.
Unlike model organisms with large collections of mutant strains to draw upon, the lack of reverse genetic tools in the mosquito has made it is very difficult to assign functions to genes in a definitive manner," Adelman said.
Several reverse genetic strategies have been successfully used for functional genomics in animal and plant species including chemical mutagenesis (3).
cells destroyed by Alzheimer's disease, reverse genetic defects such as cystic fibrosis, and re-grow severed spinal cords.
The editors (both of the Centre for Crop Genetic Improvement, Rothamsted Research, UK) believe that the production and characterization of transgenic plants is a powerful reverse genetic strategy that can be used in cereals research to ascribe function to defined DNA sequences.
This could someday be used to reverse genetic defects, cure disease or delay aging.
Whether the deletion in the ORF of NSP 3b is involved in pathobiologic changes should be assessed with reverse genetic systems.
The upshot of all this, apparently, is that Brian Boru is "tail-male and tail-female to near reverse genetic relatives".
1981), and targeted gene disruption (Rong and Golic 2000) have allowed researchers to conduct molecular and reverse genetic analyses.
Now, using what is called a reverse genetic approach, he and his colleagues have been able to demonstrate the protein is exactly what they thought.
PART III FORWARD AND REVERSE GENETIC SYSTEMS FOR DEFINING VIRULENCE