reverse genetics

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reverse genetics

a conceptual approach allowing the study of gene function; it can be summarized as "from gene to phenotype" as opposed to forward genetics, which can be summarized as "from phenotype to gene." It uses structural changes in the gene by deletion through gene knockout or disruption by site-directed mutagenesis, and then investigating the effects of such alterations on the phenotype of the cell or organism.
Synonym(s): positional cloning

reverse genetics

methods such as antisense nucleic acids and site-directed mutagenesis that are used to selectively study gene function. Contrasts with classical genetics which depends on the isolation and analysis of cells (animals) with random mutations that can be identified.
References in periodicals archive ?
These illustrations provide information for both forward (phenotype to genotype) as well as reverse genetics (genotype to phenotype) approaches.
Reverse genetics of RNA viruses; applications and perspectives.
PART III FORWARD AND REVERSE GENETIC SYSTEMS FOR DEFINING VIRULENCE
elegans as an in vivo model and apply large-scale forward and reverse genetic screenings with high-throughput.
After general overviews, the sections cover population genetics and evolutionary approaches, forward and reverse genetic systems for defining virulence, and comparative "omics" approaches to defining virulence.
Using reverse genetic engineering, scientists have discovered that the 1918 influenza pandemic that killed fifty million people worldwide was a form of this virus.
Whether the deletion in the ORF of NSP 3b is involved in pathobiologic changes should be assessed with reverse genetic systems.
To this end the researchers performed a reverse genetic loss-of-function screen with a small interfering RNA (siRNA) library representing 160 kinases.
By integrating diverse genomic-scale analyses and using reverse genetic, we identify novel DG proteins that are singularly exported beyond the tachyzoites-hosting PV to the host cell nucleus, thus extending the scope of the function of DG proteins beyond their dedicated role in vacuole formation.
Specific topics encompass the paleobotany of Livingston Island, Antarctica; targeted isolation and sequence assembly and characterization of the white spruce; the complete chloroplast genome sequence of a tree fern; reverse genetic analyses of gene function in fern gametophytes; glossopterid seed ferns from the Late Permian period; pollen development; DNA barcoding in the Cycadales; expressed sequence tag analysis in Ginkgo biloba; frequent fires in ancient shrub tundra; the history of native plant communities in the South; past vegetation patterns of New Mexico's Rio Del Oso Valley; and East Asian monsoon and paleoclimatic data analysis.
This highly infectious H5N1 strain is reassortanted with a human influenza virus strain through reverse genetic technology; which is used to imitate the potential pandemic strain.
Reverse genetic studies under way in our laboratory are designed to assess this possibility.