restriction site

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re·stric·tion site

a site in nucleic acid in which the bordering bases are of such a type as to leave them vulnerable to the cleaving action of an endonuclease.
Synonym(s): cleavage site

re·stric·tion site

(rĕ-strik'shŭn sīt)
A site in nucleic acid in which the bordering bases are of such a type as to leave them vulnerable to the cleaving action of an endonuclease.
Synonym(s): cleavage site.

restriction site

the specific nucleotide site recognized by restriction endonuclease. Called also restriction endonuclease site.

restriction site bank

Patient discussion about restriction site

Q. Is anyone restricted to have barley? what is the benefit of having barley and what is the best way to consume them? Is anyone restricted to have barley?

A. It grows in many parts of the world. As it is a whole grain it is good for health. It has soluble fiber and reduces blood cholesterol and glucose. It is low in fat content. No fixed way is there to eat barley as it’s used as soup thickener; it’s used in baked foods. Many breakfast foods include barley as baked breads. It is found to harm none.

Q. Is exercise recommended during pregnancy, if yes, are there any restrictions during pregnancy? I am in my 13 weeks of pregnancy. I always try to keep me fit and I do cycling every morning, swimming and yoga. These days I feel my body is changing and I am feeling more tiresome and nauseated. Is exercise recommended during pregnancy, if yes, are there any restrictions during pregnancy?

A. I exercised all through my pregnancies. I only gained a total of 10-12 pounds each time. I had easy deliveries because of the exercise. This has nothing to do with nausea. I had 9 months of nausea the first time around. Don't overdo the exercise, walking is the best exercise ever, and I climbed hills and stairs and walked several miles a day. My shortest delivery time was 5 minutes. I almost did not make it to the hospital. All my babies were healthy.

Q. What actions should i take in order to keep my self in a sharp and restricted fitness control?

A. I would try some body weight circuits 3 to 4 times a week.

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References in periodicals archive ?
A general advantage of bisulfite-based methods is that they are not limited to the evaluation of methylation at restriction sites only; all types of CpG methylation can be studied.
Haplotypes of the two clusters differed by five or more restriction sites, and the average nucleotide divergence between individual haplotypes in the two clusters averaged 2.
8 kb of Pu DNA subcloned into the EcoRI restriction site within the polylinker region of pBluescribe (Stratagene) (McLean et al.
Despite the uncertainty in some of the subgenus assignments,(2) our analyses of mtDNA restriction sites show some concordance with subgeneric assignments.
PCR products must not contain additional restriction sites for the enzymes used (information provided by our program DRMP_V0.
606-979)] was assembled first by generating a GAD65 PCR fragment (coding for amino acids 234-442) with the restriction sites BamHI at the 5' end and StuI at the 3' end.
The G[right arrow]A transition at position -800 was detected by the loss of the restriction site for MaeIII: the G allele is defined by the presence of two polymorphic bands of 205 and 137 bp, whereas the A allele is defined by the absence of the MaeIII site and is detected by a 342-bp band.
This enzyme did not reveal polymorphisms in CYP21P in the samples examined in this study; however, we demonstrated that there are two polymorphic HaeIII restriction sites in this intron, one located around nt398, and one located around nt509.
The frequencies of the HaeIII and BbvI polymorphisms, which fall on restriction sites, were determined in DNA samples from 24 Centre d'Etude du Polymorphisme Humain (CEPH) subjects in the parental generation (48 chromosomes).
Methods for detection of point mutations, such as ASO hybridization (9,19, 20), amplification-created restriction sites (24, 25), or ligase chain reaction (23), can only find mutations already known.
Four of the mutations eliminate restriction sites, one mutation creates a new site, and one mutation changes the location of a restriction site (Table 1).