restriction fragment

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restriction fragment

a fragment of DNA produced by cleavage by a specific endonuclease.
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The selected and interpolated traces were analyzed with custom software to identify restriction fragment maps for the sample.
Resulting terminal restriction fragment (TRF) profiles were standardized for analysis using procedures similar to those described by Dunbar et al.
granulosus on the basis of random amplification of polymorphic DNA-PCR (RAPD-PCR) and polymerase chain reaction based restriction fragment length polymorphism (PCR-RFLP) to identify internal transcribed spacer genel (ITS1) variant.
These techniques include karyotyping, random amplification of polymorphic DNA, restriction fragment length polymorphism (RFLP), DNA hybridization studies, amplified fragment length polymorphism (AFLP), and polymerase chain reaction (PCR) fingerprinting (12-17).
The genotypes for three restriction fragment length polymorphisms of the VDR were determined by polymerase chain reaction (PCR) (Techne Gradient, Camridge, UK) and enzymatic digestion of the products with BsmI, ApaI and TaqI restriction enzymes.
Many HCV genotyping methods have been developed, including restriction fragment length polymorphism analysis, genotype-specific PCR, heteroduplex mobility analysis, melting curve analysis with fluorescence resonance energy transfer probes, and line probe assay, a DNA hybridization method; however, nucleotide sequencing of an appropriate subgenomic region remains the most widely accepted method (13-18).
Restriction fragment analyses of PCR-amplified regions of mtDNA provide a rapid and practical method for detecting nucleotide sequence variation in mtDNA between individuals or species.
Restriction fragment length polymorphism analysis detecting a community-based tuberculosis outbreak among persons infected with human immunodeficiency virus.
These were CS33 (Figure 1, lane 8) and MH222 (data not shown), which both had restriction fragments at 175 and 50 bp and additional variant bands at 65 and 70 bp, respectively.
The RE was scored as inactive or as having low, moderate, or high activity based on the number of restriction fragments produced.
Isolation of plasmid DNA, cleavage of restriction fragments, and purification of DNA fragments from agarose type VII (Sigma Chemical Co.

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