restriction digest


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restriction digest

a unique family of DNA fragments produced by digestion of a given DNA molecule by a particular restriction enzyme; usually visualized by agarose gel electrophoresis and ethidium bromide or other fluorescent procedures.
References in periodicals archive ?
With the array, any reference sample can be used for analysis without changes to the standard aCGH protocol, and no restriction digest is required.
The restriction digest products were electrophoresed on 2% agarose gel in 1X TAE buffer and PCR-RFLP bands were visualized under UV light by ethidium bromide staining.
To investigate the fragment length-dependent sequencing bias, we prepared a restriction digest of [lambda] DNA (Invitrogen).
Our PCR, restriction digest, and electrophoresis protocols were adapted from Gruntzig et al.
1997), when applied properly, differentiates domestic cats from cougars based on examining the RsaI restriction digest.
Four distinct phylogenetic lineages of human serotype III GBS have been identified by PFGE of restriction digest patterns and designated restriction digest patterns type III-1, III-2, III-3, and III-4 (11).
Fragment lengths were estimated using a HaeIII digest of [phi]X174RF (New England Biolabs) and an appropriate restriction digest of strain 1A2 as lane markers.
To quantify the proportion of the DNA that could be digested, we divided the density of the full-length PCR product after the restriction digest by the density before the restriction digest.
pseudomallei were typed by PFGE of an XbaI restriction digest of bacterial chromosomal DNA (Figure).
The mean mitochondrial genome size was calculated by averaging the sum of the individual fragments for each restriction digest (Table 1).
An aliquot of the restriction digest is taken for PFGE analysis as a control for DNA preparation; the remaining prepared DNA molecules are analyzed with the DLA system.