restriction fragment length polymorphism

(redirected from Restriction Fragment Length Polymorphisms (RLFPs))
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re·stric·tion frag·ment length pol·y·mor·phism (RFLP),

used in genetic analysis of populations or individual relationships. In regions of the human genome not coding for proteins, the distance (in nucleotides on the chromosome) can be different between two genetic markers, usually because of repeated base patterns.

restriction fragment length polymorphism (RFLP)

a difference in the DNA sequences of homologous chromosomes as revealed by different lengths of the restriction fragments produced by enzymatic digestion of a selected region of chromosomes. RFLPs are believed to be inherited according to mendelian laws and have been used to locate the genes associated with several inherited disorders, including Huntington's disease.

re·stric·tion frag·ment length pol·y·mor·phism

(RFLP) (rĕ-strik'shŭn frag'mĕnt length pol'ē-mōrf'izm)
Used in genetic analysis of populations or individual relationships. In regions of the human genome not coding for proteins there is often wide (but measurable) sequence variety between people.

restriction fragment length polymorphism (RFLP)

Variations within a species in the lengths of fragments of DNA caused by RESTRICTION ENZYMES. The variations are caused by mutations that either abolish the normal sites of breakage or create new ones, characteristic of the mutations. RFLP analysis may allow genetic abnormalities to be detected, often before birth, even if the location of the mutated gene or genes is unknown.

restriction fragment length polymorphism (RFLP)

a MUTATION that results in a change in the pattern of restriction fragments generated when a DNA molecule is treated with a RESTRICTION ENZYME (see RESTRICTION ANALYSIS). The RFLP is apparent when the fragments are resolved by AGAROSE GEL ELECTROPHORESIS. RFLPs can be used as reference LOCI for mapping, in relation to specific linked genes (see GENETIC LINKAGE). RFLPs can be used as a diagnostic test for genetic DISEASES, by detecting specific gene defects. The presence of the RFLP may be a direct indication of the gene defect, or may be closely linked to it. Diseases that have heen diagnosed by RFLP analysis include certain types of THALASSAEMIA and HAEMOPHILIA, and by RFLP linkage analysis, HUNTINGTON'S CHOREA.
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