Scientists have believed for a number of years that a regulatory element
could control a gene by physically interacting with that gene, Mr.
Abbreviation Enhancer -- Repressor/silencer -- Insulator -- MicroRNA miRNA Small interfering RNA siRNA Telomere-specific small RNA tel-sRNA Piwi-interacting RNA piRNA Promoter-associated small RNA PASR Enhancer RNA eRNA Regulatory function Composition Increase transcription DNA sequence Decrease transcription DNA sequence Shield genes from distal inhibitory signals DNA sequence Decrease translation, increase mRNA degradation Small ncRNA Increase mRNA degradation Small ncRNA Regulate cell cycle Small ncRNA Repress retrotransposons Small ncRNA Regulate transcription Small ncRNA Increase transcription ncRNA
The gene regulatory element
and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
The gene that Penninger's team had mutated encodes a protein called DREAM (downstream regulatory element
antagonistic modulator), which is a so-called transcription factor.
The construct includes a glucose responsive regulatory module having at least one glucose inducible regulatory element
comprising a pair of CACGTG motifs linked by a five base nucleotide sequence, which confers glucose inducible expression of the proinsulin coding sequence.
netCEP(SM) provides both individuals and Member Firms secure, on-demand, Internet access to a library of instructional materials that serve as a resource for Regulatory Element
preparation, Firm Element planning and general rule, product and compliance training.
The methods generally comprise introducing into a genome of an animal a genetic construct comprising a transcriptional regulatory element
operably linked to a heterologous marker gene encoding a marker, wherein the element drives expression of the marker across genera transgenic in the construct sufficient to visually detect the marker in photoreceptive cells or organs, and selecting for transgenesis by visually detecting the marker in a photoreceptive cell or organ of the animal.
University of California (Oakland, CA) has patented a recombinant nucleic acid molecule containing an inducible regulatory element
operably linked to a nucleic acid molecule encoding a floral meristem identity gene product such as APETALA1, CAULIFLOWER or LEAFY.
Rhone-Poulenc Agrochimie (Lyons, France) has patented an isolated DNA sequence capable of serving as regulatory element
in a chimeric gene which can be used for the transformation of plants.
The compound also targets the structurally similar quadruplex regulatory element
of VEGF (vascular endothelial growth factor), a validated target of other cancer drugs including Avastin(TM).
In addition, CIStem is applying its technology to regulatory element
activity profiling (REAP(TM)), a novel assay for testing drug compounds for important activities, such as mechanism of action and toxicity.
Gene Logic is the clear and proven leader in the application of gene expression analysis to complex diseases, and is the only group capable of very high throughput characterization of gene regulatory element
and transcription factors.