RT-PCR


Also found in: Acronyms.

RT-PCR

RT-PCR

Abbreviation for reverse transcriptase polymerase chain reaction.

Reverse transcription-polymerase chain reaction; RT-PCR

A method of polymerase-chain-reaction amplification of nucleic acid sequences that uses RNA as the template for transcribing the corresponding DNA using reverse transcriptase.
Mentioned in: Noroviruses

RT-PCR

reverse transcriptase-polymerase chain reaction. See PCR1.
References in periodicals archive ?
During 2016, tissue specimens from 627 completed pregnancies with possible maternal Zika virus exposure from the 50 states and DC were submitted to CDC and were tested by Zika virus tissue RT-PCR.
The most recent RT-PCR for Zika virus was performed for breast milk 33 days after onset of signs and symptoms and 9 days after delivery.
Briefly, 5 u l of undiluted first RT-PCR product was added to a reaction mixture consisting of 3 u l of 25mM MgCl2, 2 u l of 10mM dNTPs, 0.
Considering that the evidence on the validity of RT-PCR in TBP is insufficient, we decided to carry out a study to evaluate the validity of this test comparing it with a combined reference method, in the adult hospitalization service of the Hospital Universitario de la Samaritana (HUS), in patients with lymphocytic exudate pleural effusion (according to light criteria and with a higher proportion of lymphocytes than neutrophils).
Of the 24 women with positive RT-PCR results and adverse pregnancy outcomes, 23 had symptom onset during the first trimester, while all 8 patients with positive RT-PCR results but healthy infants had symptom onset in the third trimester (P less than .
In a study by Wang et al in 2010 dengue infection was detected by either MAC-ELISA or RT-PCR in 300 samples (93.
In conjunction, the company performs testing for its proprietary Zika RT-PCR test as well as the CDC-based Zika MAC-ELISA test at its Focus Diagnostics laboratory in San Juan Capistrano, California as well as performs the molecular RT-PCR Zika test for use on serum and paired urine specimens at its laboratory in Chantilly, Virginia.
CDCs Enterovirus D68 2014 Real-time RT-PCR Assay (EV-D68 2014 rRT-PCR) for the in vitro qualitative detection of RNA from the Enterovirus D68 (EV-D68) strains detected in North America In 2014 in upper respiratory specimens (such as nasopharyngeal [NP] swabs, oropharyngeal [OP] swabs, dual NP/OP swabs, and/or nasal washes) and sera in conjunction with patient-matched upper respiratory specimen(s) from individuals with signs and symptoms of EV-D68 infection and/ or epidemiologic risk factors, by qualified laboratories designated by CDC on specified instruments.
Gilles Martin, Eurofins CEO said, As a leader and trusted laboratory partner specialized in and dedicated to improving patient care through innovative infectious disease testing, Viracor-IBT is thrilled to be one of the first commercial laboratories to offer the Zika RT-PCR test to hospitals and health systems, and we are pleased that the FDA recently authorized our assay.
The aim of present study was to isolate and identify the IBD virus from field samples by using different techniques, serological method agar gel precipitation test and molecular method using RT-PCR to diagnose IBDV from bursal field samples.
RT-PCR product was purified, send to company for sequence(Takara Bio Inc.
The finding says, "The RDT detected all confirmed cases of EVD that were positive by RT-PCR in both point-of-care (28/105 patients) and laboratory testing (45/277 patients), with sensitivity of 100%.