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RT-PCR

   Also found in: Acronyms 0.03 sec.
Reverse transcription-polymerase chain reaction; RT-PCR
A method of polymerase-chain-reaction amplification of nucleic acid sequences that uses RNA as the template for transcribing the corresponding DNA using reverse transcriptase.
Mentioned in: Noroviruses

RT-PCR
reverse transcriptase-polymerase chain reaction. See PCR1.


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Although the initial capital equipment expenditure is high: * The platform can be used to test for numerous pathogens and can be quickly adapted to novel pathogens; * Many RT-PCR assays can be manufactured "in house", thus reducing cost and assuring availability in a time of international crisis; * The majority of these reagents can be stored for years, facilitating stockpiling.
On the basis of experiences during the outbreak of severe acute respiratory syndrome (SARS) in 2003 (7), an ENIVD member laboratory distributed a then-unpublished real-time RT-PCR protocol that had been evaluated with a large number of clinical samples from imported cases to laboratories asking for assistance (3).
To unravel the role of Vitamin D3 in BC patients, we have investigated serum levels of 25(OH)D3 and its active form 1,25(OH)2D3 as well as tissue expression of 1alpha-hydroxylase, 24-hydroxylase, and Vitamin D-receptor (VDR), determined by semiquantitative RT-PCR, in 88 Brazilian BC patients and 35 women without cancer (submitted to mammoplasties or resection of benign lesions).
 
 
 
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