ROCK1

ROCK1

A gene on chromosome 18q11.1 that encodes a protein kinase, which is a key regulator of actin cytoskeleton and cell polarity. It regulates smooth muscle contraction, actin cytoskeleton organisation, stress-fibre and focal adhesion formation, and neurite retraction, as well as cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. It phosphorylates FHOD1, and both promote SRC-dependent non-apoptotic plasma membrane blebbing. ROCK1 also: phosphorylates JIP3 and regulates JNK to JIP3 recruitment in response to UVB-induced stress; suppresses inflammatory cell migration by regulating PTEN phosphorylation and stability; downregulates VEGF-induced angiogenic endothelial cell activation; promotes keratinocyte terminal differentiation; and plays a role in terminal erythroid differentiation. ROCK1 is required for centrosome positioning and centrosome-dependent exit from mitosis. It may regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles.
References in periodicals archive ?
MicroRNA-148a promotes myogenic differentiation by targeting the ROCK1 gene.
Arabidopsis ROCK1 transports UDP-GlcNAc/UDP-GalNAc and regulates ER protein quality control and cytokinin activity.
MicroRNA-1280 inhibits invasion and metastasis by targeting ROCK1 in bladder cancer.
miRNAs Blanco asociado Funcion del miRNA en metastasis de tumor miR-10b HOXD10 Promotor miR-21 PTEN, PDCD4, TPM1, Maspin Promotor miR-373 CD44, LATS2 Promotor miR-520c CD44 Promotor miR-155 RhoA Promotor Let-7 Ras, HMGA2 Represor miR-335 SOX4, TNC Represor miR-126 Crk, IRS-1 Represor miR-146a ROCK1 Represor miR-101 EZH2 Represor Familia miR-200 ZEB1, ZEB2 Represor
Michael Armstrong Professor of Medicine at the Johns Hopkins University School of Medicine, said that high levels of RhoA and ROCK1 were known to worsen outcomes for breast cancer patients by endowing cancer cells with the ability to move, but the trigger for their production was a mystery.
MicroRNA-148a suppresses tumor cell invasion and metastasis by downregulating ROCK1 in gastric cancer.
Similarly, the ROCK (Rho-associated protein kinase) inhibitor may have significant patient value because expression of its target ROCK1 is significantly higher in breast tumor tissue than normal tissue and is associated with aggressive disease, the spread of cancer cells and poorer prognosis.
Finally, MRCKa has two closely related family members, MRCKb and MRCKg, and also shares functional similarities with ROCK1 and ROCK2.
The primer sequences were as follows: ROCK1, forward 5'-TGGTTGGGACGTACAGTAAAA-3' and reverse 5'-GTAAGGAAGGCACAAATGAGA-3'; ROCK2, forward 5'-AATGGGTTAGTCGGTTGG-3' and reverse 5'-CTTGGTTTGTTTGGAGCA-3'; eNOS, forward 5'-GCCAAACAGGCCTGGCGCAA-3' and reverse 5'-GTGCTGTCCTGCAGTCCCGA-3'; PKG-I, forward 5'-CTTGGAGTGGGAGGTTTC-3' and reverse 5'-AATGTGTCGTTTCTTGAGG-3'; ICAM-1, forward 5'-TATCCATCCATCCCACAG-3' and reverse 5'-GTTCGTCTTTCATCCAGTTAGT3'; [beta]-actin, forward 5'-TACAACTCCTTGCAGCTCC-3' and reverse 5'-ATCTTCATGAGGTAGTCAGTC-3'.
miR146, which is expressed at lower levels in androgen-independent cell lines, targets ROCK1 [encoded by rho-associated, coiled-coil containing protein kinase 1 (ROCK1)], a key kinase in hyaluronan-mediated transformation to androgen independency (45).
Hydroxyfasudil-mediated inhibition of ROCK1 and ROCK2 improves kidney function in rat renal acute ischemia-reperfusion injury," PLoS ONE, vol.
Podocyte foot process effacement also has been observed under high glucose conditions, probably through phosphorylation of Drp-1 by ROCK1 [75].