RNase T1 | definition of RNase T1 by Medical dictionary
a nuclease that endonucleolytically cleaves ribonucleic acids at the 3'-5' link of a guanosine 3'-phosphate residue, producing oligonucleotides terminating in this nucleotide; a transferase (endonuclease) in the first (cyclizing) step, a phosphodiesterase on the second (hydrolyzing) step.
Synonym(s): guanyloribonuclease, RNase N1
References in periodicals archive
This was resuspended in TE buffer, digested (37 [degrees] C, 30 min) with RNase A and dialyzed RNase T1
(Sigma-Aldrich), followed by addition of 10% sodium dodecyl sulfate and proteinase K for 30 min more.