The qPCR included primers and probe for an attenuated amplification of the RNase P gene RPPH1.
Regression curves for each of the TREC and RNase P calibrators (Fig.
2) demonstrated separation between each of the TREC calibrators when viewed alone and appropriate attenuation of the RNase P when viewed together.
Tests of DBS calibrators made from dilutions of known leukocyte concentrations into leukocyte-depleted blood (14) showed RNase P copy numbers consistent with expected concentrations of cells per milliliter (e.
RealTime PCR systems TegMan RNASE P
96-WELL m2000rt Instrument Verification Plate
The Company's technologies utilize either External Guide Sequences (EGS) oligozymes - which inactivate targeted messenger RNA (mRNA) through use of cellular enzyme RNase P
- or RILON(TM) oligozymes, which inactivate targeted mRNA directly.
Once the EGS molecules attach to their target, they cause a cellular enzyme called RNase P
to destroy the mRNA to which they are bound; the EGS molecules are then freed to repeat the process.
Goldberg, in a talk entitled "Harnessing the Ribozyme RNase P
to Develop Therapeutics," discussed several key advances in the Company's research.
EGSs are not ribozymes, but short oligonucleotides that redirect the activity of RNase P
to target and destroy disease-causing RNA before they can be used to create disease-causing proteins.
Cashmore and his colleagues in plant cell research should enable us to gain valuable information about the effects of EGSs and their interaction with the RNase P
enzyme in plant systems," said Dr.
External Guide Sequences (EGSs) are small oligonucleotides that can be designed to bind to disease-causing RNA, resulting in its inactivation through the cutting activity of RNase P
, an enzyme present in all cells.
EGSs are not ribozymes but short oligonucleotides that redirect the activity of the ribozyme RNase P
, an enzyme found in every living cell, to target disease-causing RNAs.