As discussed, in rhPCR, a mismatch at or near the RNA base inhibits the ability of RNase
H2 to unblock an rhPCR primer.
Hence, we investigated their ability to inhibit both HIV-1 RT-associated RNase
H and RDDP functions using the RNase
H selective diketo acid (DKA) derivative DS1759 (Corona et al.
Nucleoside modifications in RNA limit activation of 20-50-oligoadenylate synthetase and increase resistanc to cleavage by RNase
In this case, the possibility of the presence of active RNase
during surgery possibly led to RNA degradation and could not be ruled out.
To prevent RNases
from degrading the RNA probe, a careful work method is necessary, and all buffers and solutions should be prepared with RNAase-free ultrapure water as far as possible.
In order to elucidate the mechanism of interaction of RNase
Optimization of the media constituents for overproduction of RNase
by Aspergillus niger was done by central composite experimental design (CCD), where a [2.
The TREC and RNase
P values were similar to those observed with the routine DNA preparation.
Strong nucleic acid binding to the Escherichia coli RNase
HI mutant with two arginine residues at the active site.
Given the importance of the surrounding environment for protein stability, we assessed the denaturation profiles for 10 [micro]g RNAse
A using 20 mM Tris (prt 7.
Cheney hypothesizes that CFS moves through three distinct stages that begin when an intracellular organism activates the RNase
Parniak, Professor of Molecular Genetics & Biochemistry at the University of Pittsburgh and Principal Investigator of this multi-team research effort, has nearly 20 years experience in HIV research and is regarded as a thought leader in the discovery of drugs targeting HIV RNase