We analyzed the samples for RHDV by reverse transcription PCR.
Retrieved sequences represent the RHDV groups G1-G6, the nonpathogenic groups, and the new variant (GenBank accession nos.
The RHDV-N11 sequence was shown to form a branch falling between RCV and RCV-A1, separated from RHDV and RHDVa (Figure 1).
A major band of [approximately equals] 60 kDa was observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and detected by Western blot by using a rabbit polyclonal antibody against RHDV Ast/89 (4).
Agglutination studies showed that RHDV Ast/89 agglutinated human blood of all group types (O, A, B, and AB), as described for RHDV genetic group 1 members (73).
To determine if human epithelial cells were recognized by RHDV, binding of the G3 strain to human tissue sections was assessed.
This finding indicated that attachment factors for RHDV are present on human cells that constitute potential points of entry for RHDV (online Technical Appendix Figure, panels B-E).
In this study, we have shown that cross-species recognition of HBGAs in cells that may be likely points of entry of RHDV into human cells.
Results from inoculation testing and RT-PCR using standard primers (3) within the RHDV polymerase region at FADDL were not consistent with RHD, and RHDV was ruled out as the cause of the outbreak.
RHDV was immunohistochemically detected within the cytoplasm of approximately 20% of hepatocytes in 1 of the initially submitted does, primarily in the periportal and midzonal areas (Figure 5).
It also highlights the importance of using wildlife monitoring schemes as detection tools for monitoring the impact of stochastic factors, such as the variant RHDV, on wildlife populations.
Spread of new variant of RHDV in domestic rabbits on the Iberian Peninsula.