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RFLP

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RFLP
abbr.
restriction fragment length polymorphism

restriction fragment length polymorphism (RFLP),
a difference in the DNA sequences of homologous chromosomes as revealed by different lengths of the restriction fragments produced by enzymatic digestion of a selected region of chromosomes. RFLPs are believed to be inherited according to mendelian laws and have been used to locate the genes associated with several inherited disorders, including Huntington's disease.

RFLP
restriction fragment length polymorphism.

RFLP
Restriction fragment length polymorphism Molecular medicine A local variation in the DNA sequence of an individual which may be detected by restriction endonucleases–which 'cut' the double-stranded DNA whenever they recognize a certain highly-specific oligonucleotide sequence or 'restriction' site. See DNA, DNA sequence, Linkage map, Marker, Mutation, Physical map, Polymorphism.


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2), lane 2 shows the fragment patterns produced from an individual whose RFLP phenotype with both enzymes did not agree with its morphological identification as La.
To identify larvae of these species, we developed species-specific polymerase chain reaction (PCR) and RFLP assays based on the internal transcribed spacer (ITS) regions of the ribosomal gene complex.
 
 
 
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