RFLP


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RFLP

restriction fragment length polymorphism (RFLP)

a difference in the DNA sequences of homologous chromosomes as revealed by different lengths of the restriction fragments produced by enzymatic digestion of a selected region of chromosomes. RFLPs are believed to be inherited according to mendelian laws and have been used to locate the genes associated with several inherited disorders, including Huntington's disease.

RFLP

Restriction fragment length polymorphism Molecular medicine A local variation in the DNA sequence of an individual which may be detected by restriction endonucleases–which 'cut' the double-stranded DNA whenever they recognize a certain highly-specific oligonucleotide sequence or 'restriction' site. See DNA, DNA sequence, Linkage map, Marker, Mutation, Physical map, Polymorphism.

RFLP

Abbreviation for restriction fragment length polymorphism.

RFLP

abbrev. RESTRICTION FRAGMENT LENGTH POLYMORPHISM.

RFLP

restriction fragment length polymorphism.
References in periodicals archive ?
The RFLP results for the PCR product (574bps) of Mycoplasma mycoides sub-cluster members (Mmc and Mmm SC) yielded three bands (fragments) of 230, 178, and 153bps specifically for Mmc when digested with Vsp1 (Fig.
Nakon RFLP secenja, HhaI enzimom na finom MetaPhore 4% uoceni su fragmenti od 91 bp, 48 bp i 35 bp kod prvog subjekta.
This study thus suggests that this detection method of PCR along with RFLP using PvMSP3[beta] gene as a marker is suitable for genotyping P.
After initial establishment, RFLP analysis was used to assess the copy number of IS6110 in M.
An AluI PCR RFLP detecting a silent allele at the goat POU1F1 locus and its association with production traits.
25,30) We further classified the samples into two groups (positive by both procedures versus positive only by nested-PCR) and observed different patterns of HPV types and frequency by RFLP and sequencing.
It does however suggest that the COL5A1 BstUI RFLP, as a risk factor for musculoskeletal soft-tissue injuries, is not age dependent in females.
8) 30 BCC + 9 from other specimens Year of isolation (PGIMER) Genomospecies RFLP & source 2002 (identified by API 20 NE) 2004 (Nov, 2004-Jan 2005) 2005 1 - B.
Each time we encountered a discrepancy between RFLP and morphological identification, we sequenced the DNA of the PCR product from the individual in question.
To identify larvae of these species, we developed species-specific polymerase chain reaction (PCR) and RFLP assays based on the internal transcribed spacer (ITS) regions of the ribosomal gene complex.