PCR1

(redirected from Quantitative PCR)
Also found in: Acronyms.

PCR1

polymerase chain reaction. The amplification of a specific DNA sequence, termed target or template sequence, that is present in a complex mixture, by adding two or more short oligonucleotides, also called primers, that are specific for the terminal or outer limits of the template sequence. The template-primers mixture is subjected to repeated cycles of heating to separate (melt) the double-stranded DNA and cooling in the presence of nucleotides and DNA polymerase such that the template sequence is copied at each cycle. Thermostable polymerases such as those obtained from a hot springs bacterium Thermus aquaticus, commonly termed Taq polymerase, are used.
At the end of 20 to 30 such cycles, the amplified target sequence, which may have been present in as few as a single copy in the original mixture, can be readily detected, for example by electrophoresis and ethidium bromide staining in an argarose gel.

multiplex PCR
the detection of more than one template in a mixture by addition of more than one set of oligonucleotide primers.
nested PCR
the primers used in the first round of amplification are either both replaced (nested PCR) or only one is replaced (semi-nested PCR) for the second and subsequent cycles of amplification. Increases the sensitivity and specificity of the PCR.
quantitative PCR
a means for quantifying the amount of template DNA present in the original mixture. Usually achieved by the addition of a known amount of a target sequence that is amplified by the same primer set but can be differentiated, usually by size, at the end of the reaction.
real-time PCR
a method for the detection and quantitation of an amplified PCR product based on incorporation of a fluorescent reporter dye; the fluorescent signal increases in direct proportion to the amount of PCR product produced and is monitored at each cycle, 'in real time', such that the time point at which the first significant increase in the amount of PCR product correlates with the initial amount of target template.
reverse-transcriptase PCR (RT-PCR)
a reaction applied when the target sequence is RNA, such as viral RNA or messenger RNA. Reverse transcriptase that copies DNA from an RNA template is present in the first round.
References in periodicals archive ?
SYBR green-based real-time quantitative PCR assay for detection of West Nile Virus circumvents false-negative results due to strain variability.
PCR reaction mixtures contained 5 [micro]L of sample DNA in Platinum Quantitative PCR SuperMix UDG (Invitrogen, Carlsbad, CA), 200 nM each primer and 40 nM probe, with a reaction volume of 25 [micro]L.
To investigate the involvement of GPR43 in pig primary cultured adipocytes differentiation in vitro, GPR43, LPL and PPAR[gamma] mRNA levels were analyzed at different cultured time using quantitative PCR.
difficile in patients with ulcerative colitis and to compare this carriage with that in healthy individuals using a sensitive quantitative PCR employing primers to amplify species-specific segments of the 16S rDNA, and to reassess the rate of detection of C.
Vandemark and Miklas note that their new approach to using quantitative PCR can make such determinations in about 2 hours.
As a result, quantitative PCR results could be used to guide when antifungal therapy could be safely discontinued in an individual patient, he said.
A new, highly sensitive quantitative PCR assay detected cytomegalovirus (CMV) DNA before symptoms developed in stem cell transplant recipients, according to researchers at the University of Washington in Seattle.
The primary objective of this research is to identify trends for quantitative polymerase chain reaction (PCR) techniques, specifically the use of nascent digital PCR (dPCR) technology in comparison with the mature real-time quantitative PCR (qPCR) technology.
Quantitative Pcr Block 384 Well Microfluidic Card - 1 Piece.
The Precise assays use next generation sequencing in conjunction with Cellular Research's Molecular Indexing(TM) technology to create a powerful, more accurate alternative to quantitative PCR (qPCR).
Compared with real-time quantitative PCR (qPCR), dPCR has already been heralded as more precise (3), better at detecting rare genetic variants (4), and less susceptible to inhibitors (5, 6).
We show that this prevention in areal bone mineral density and micro-CT parameters results from the stimulation of bone formation, demonstrable in vivo by histomorphometry, bone marker measurements, and quantitative PCR.

Full browser ?