Prussian blue stain

Prus·sian blue stain

a stain employing acid potassium ferrocyanide to demonstrate iron, as in siderocytes.
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In our study exfoliated cells from the buccal mucosa of 60 [beta] thalassemia major patients comprising the study undergoing transfusions and a control group of 30 normal individuals who had no confirmed acute and chronic liver damage, malignancy and megaloblastic anemia were considered and the smears obtained were stained with Perls' Prussian blue stain.
The scraps were smeared into the centre of the glass slides & immediately fixed in 90% alcohol for one hour, then stained with Perls' Prussian blue stain (which consist of Potassium ferrocyanide and HCl) which reacts with the ferritin in the cells to form blue coloured granules.
Figure 1: Smear at higher magnification showing clumps of blue colored granules (Perls' Prussian blue stain, x 400).
Figure 2: Smear at higher magnification showing dispersed blue colored granules (Perls' Prussian blue stain, x400)
In this situation, a positive reaction with a Prussian blue stain, as opposed to one with a Fontana-Masson stain, can confirm the presence of hemosiderin.
3), and alveoli with numerous haemosiderin-containing histiocytes, and the Prussian blue stain for iron confirmed severe pulmonary haemosiderosis (Fig.
Golde has introduced a ranking score, based on the hemosiderin content of macrophages which enables ranking cells from 0 to 4 based on the degree of Prussian blue stain.
Prussian blue stain revealed abundant hemosiderin particles in these same macrophages (Figure 4), with absence of staining in the uninvolved portions of the lung parenchyma.
Prussian blue stain showed iron present mostly in macrophages.
MG bodies are stained with PAS diastase, von Kossa and Perl's Prussian blue stains.