Proteins extracted by Minute[TM] kits can be used in applications such as SDS-PAGE, immunoblottings, ELISA, IP, protein localization
, gel mobility shift assays, and more.
In this study, we aimed to define OPN3 protein localization
, its abundance in the human brain and the site of cellular locality.
These include molecular biology methods for antigen production by protein expression in bacterial cells, for epitope tagged protein expression in plant and animal cells allowing protein localization
in the absence of protein specific antibodies, and for the production of anti-peptide, monoclonal, and polyclonal antibodies.
When the target gene is expressed, GFP lights up (fluoresces), creating a visual marker of gene expression and protein localization
, via light (optical) microscopy.
We describe a bioinformatics and data mining method for evaluating diagnostic serum biomarker candidates by selecting genes and gene products that possess intrinsic protein localization
and tissue expression properties.
The database combines proprietary immunohistochemistry data, obtained by using specific antibodies to determine protein localization
in normal and diseased human tissues, with manually curated data covering more than 2,000 human genes from eight gene families.
Immunohistochemical studies demonstrated p53 protein localization
in cell nuclei of both tumors, as well as in the adjacent normal-appearing adrenal cortical cell nuclei.
They all share small conserved domains named polo-box required fort protein localization
Ubiquitination also affects proteasome-independent events such as protein localization
, activity and function.
LifeSpan's DrugTarget Database is the industry's largest resource for proprietary human protein localization
data for drug targets in eight important gene families, including G protein-coupled receptors (GPCRs), nuclear hormone receptors (NHRs), kinases, ion channels, phosphatases, proteases, phosphodiesterases, and transporters.
This new microscope can be used in multiple imaging applications, including protein localization
, protein-protein interactions, gene expression, intra-cellular ion waves, calcium puffs and sparks, FRET, co-localization, tissue section morphology and 3D reconstruction.
The Phase II project funded by the NSF will enable integration of data and information on protein localization
, molecular trafficking, and the relevant cellular machineries with Cognia's existing content.