Pickering, "Cloning of a novel prolyl 4-hydroxylase
subunit expressed in the fibrous cap of human atherosclerotic plaque," Circulation, vol.
Assembly of human prolyl 4-hydroxylase and type III collagen in the yeast pichia pastoris: formation of a stable enzyme tetramer requires coexpression with collagen and assembly of a stable collagen requires coexpression with prolyl 4-hydroxylase.
Coexpression with collagen markedly increases the half-life of the recombinant human prolyl 4-hydroxylase tetramer in the yeast Pichia pastoris.
Prolyl 4-hydroxylase catalyzes the hydroxylation of proline residues in repeating X-Pro-Gly triplets, thus forming the 4-hydroxyproline required for the correct folding of newly synthesized collagen (Kivirikko and Myllyharju, 1998; Kivirikko and Pihlajaniemi, 1998; Myllyharju, 2003).
Regulation of the amount of active prolyl 4-hydroxylase tetramer appears to be linked to synthesis of the [alpha]-subunit (Kivirkko et al.
mRNA levels for [alpha]-subunit of prolyl 4-hydroxylase and fibrillar collagens in immobilized rat skeletal muscle.
Structure and Expression of the human gene for the [alpha] subunit of prolyl 4-hydroxylase.
Structural requirements for the utilization of ascorbate analogues in the prolyl 4-hydroxylase reaction.
Inhibition by N-oxalylamino acid derivatives, first developed for use with collagen prolyl 4-hydroxylases, further implies that these hydroxylases are also dependent upon 2OG as a cosubstrate (Cunliffe et al.
Characterization of the human prolyl 4-hydroxylases that modify the hypoxia-inducible factor.
Prolyl 4-hydroxylases, the key enzymes of collagen biosynthesis.
Antibody Company Clone Dilution CAF phenotype-related proteins Podoplanin Abcam, Cambridge, UK 18H5 1:100 Prolyl 4-hydroxylase
Abcam, Cambridge, UK Polyclonal 1:200 FAP[alpha] Abcam, Cambridge, UK Polyclonal 1:100 S100A4 Abcam, Cambridge, UK Polyclonal 1:100 PDGFR[alpha] Abcam, Cambridge, UK Polyclonal 1:100 PDGFR[beta] Abcam, Cambridge, UK Y92 1:100 NG2 Abcam, Cambridge, UK NG2 1:50 TABLE 2: Clinicopathologic characteristics of patients with phyllodes tumor.