chromosome walking(redirected from Primer walking)
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Related to Primer walking: Chromosome walking
sequential isolation of overlapping sequences of DNA (that is, clones); with this procedure large regions of the chromosome can be spanned.
Synonym(s): overlap hybridization
a molecular genetic technique by which overlapping molecular clones that span large chromosomal intervals are isolated.
chromosome walkingA procedure for determining the nucleotide sequence of segments of DNA that are located on either side of a region of fully characterised (i.e., “sequenced”) DNA. Chromosome walking requires the dividing (“walking out”) of each segment of DNA into overlapping short (15–20,000) bases from a point of a known sequence; this enables one to sequence DNA far removed from a marker gene.
chro·mo·some walk·ing(krō'mŏ-sōm wawk'ing)
A process of extending a genetic map by successive hybridization steps.