DLK1

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DLK1

A gene on chromosome 14q32 that encodes a transmembrane protein  and putative tumour suppressor that plays a role in the differentiation of various cell types, including adipocytes and neuroendocrine cells.
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PPAR gamma and C/EBP alpha are the adipogenic genes that play a key role in complex transcriptional cascade during adipocyte differentiation, where as Pref-1 is highly expressed in preadipocytes and the down regulation of Pref-1 is required for adipose conversion.
Semi-quantitative RT-PCR analysis of C/EBP alpha, PPAR gamma and pref-1
2005) to measure the levels of C/EBP alpha, PPAR gamma and pref-1 and beta-actin mRNAs.
Hence we checked the expression levels of PPAR gamma, C/EBP alpha and a preadipocyte factor pref-1 to measure the adipogenesis in cells treated with different concentrations of lysine and troglitazone.
Though all the treatment could induce the adipogenesis but the expression levels of C/EBP alpha, PPAR gamma and pref-1 were different among the treatments.
The results were compared with that of the troglitazone, though the gene expression trend were similar in troglitazone and lysine treated cells but the levels of expression of C/EBP alpha were significantly higher and the pref-1 level was significantly lower in troglitazone treated cells indicating that troglitazone had better capacity to induce adipogenesis compared to lysine (Figures 2 and 3).
Though the underlying mechanism of adipogenesis in this case in unclear and yet to be studied but the PPAR gamma, C/EBP alpha, pref-1 gene expression levels and the Oil red O staining results when put together clearly indicate that the low lysine (40 mg/L) can induce better adipogenic gene expression and adipocyte differentiation, thus increasing the adipogenic potential compared to the higher concentration (320 mg/L) of lysine in bovine i.