The advantage of DeNovoX is its ability to identify any kind of unknown modification of a protein with a high degree of confidence, whether the result of a single amino acid deletion in the peptide chain, a mutation, or a post-translational modification
, such as phosphorylation, resulting from some type of cellular processing.
Other features that make the 6340 an ideal tool for the study of post-translational modifications
include the ability to alternate between ETD and collision induced dissociation (CID) modes of fragmentation from scan to scan, which helps to pinpoint the precise identity and location of a chemical modification.
However, the exact function of these large numbers of post-translational modifications
is unknown (personal communication with Baker M, author of the largest human sperm proteome published to date (25)).
Acetylation is one of the most important post-translational modification
of proteins determining the structure, function and intracellular localization that plays an important role in the signal transduction pathways related to diverse cell functions, both during unstimulated and stress conditions.
Advances in MS techniques for proteome analysis have made this tool an excellent choice for the identification and quantification of proteins and post-translational modification
of proteins, with a high level of specificity and sensitivity.
I will determine the composition of evicted versus retained nucleosomes in terms of histone variants and post-translational modifications
, by performing immunoprecipitation coupled to mass spectrometry.
To advance those efforts, scientists must analyze different species of this protein - resulting from splicing or post-translational modifications
- to name those implicated in the pathological accumulation and create therapeutics with greater sensitivity and specificity.
Because plants are able to produce recombinant proteins the same way mammalian cell cultures do, including post-translational modifications
like disulfide bonds among others.
Their topics include mass spectrometry of amino acids and proteins, the structure and activity of nitrogen-methylated peptides, local surface plasmon resonance and electrochemical biosensing systems for analyzing functional peptides, atomic force microscopy of proteins, the role of cysteine, quantitative mass spectrometry-based proteomics, and bioinformatics tools for detecting post-translational modifications
in mass spectrometry data.
The CB1000 system measures phosphorylation events and other protein post-translational modifications
using capillary-based nanofluidic immunoassays, and builds upon the fundamental technology of its predecessor, the Firefly(TM) 3000 system.
An added complexity is that post-translational modifications
such as glycosylation are not genetically encoded and are therefore not identically reproduced.
of proteins; tools for functional proteomics, 2d ed.