polyvinylidene fluoride

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polyvinylidene fluoride (PVF2)

[-vīnil′idēn]
a commonly used piezoelectric material in a hydrophone. It is also used in imaging transducers.
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Subsequently, we subjected the r-survivin sample to 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by transfer to a polyvinylidene difluoride membrane.
Recombinant livin protein was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride membrane (Millipore).
Sera were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with or without 2-mercaptoethanol (2-ME), and electrophoretically transferred to polyvinylidene difluoride (PVDF) membranes (12).
Recombinant survivin protein was separated by 100 mL/L sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride membrane (Milipore).
The proteins were transferred to ProBlott polyvinylidene difluoride (PVDF) membranes (PE Biosystems) at 2 mA/[cm.
Samples were electrophoresed on 4-20% sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) minigels (31) with subsequent electrotransfer to PSQ-grade polyvinylidene difluoride membranes (Sigma), using a three-buffer semidry blotting system (32)(ESA); the stained bands were Edman sequenced (31).
After isoelectric focusing, a sheet of polyvinylidene difluoride (PVDF) membrane Immobilon (Millipore) soaked with Tris-glycine buffer (25 mmol/L Tris, 192 mmol/L glycine) was laid on the cellulose acetate membrane and left for 30 min at room temperature.