polyvinylidene fluoride

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polyvinylidene fluoride (PVF2)

[-vīnil′idēn]
a commonly used piezoelectric material in a hydrophone. It is also used in imaging transducers.
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1995) New methods using polyvinylidene difluoride membranes to detect enzymes involved in glycosphingolipid metabolism.
For SDS-PAGE and Western blot, whole bacterial cell extracts or flagella extracts were denatured and separated in 14% polyacrylamide gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore Corp.
After SDS-PAGE, the separated proteins were then transferred onto polyvinylidene difluoride membranes (Millipore Co.
The gel was transferred to a polyvinylidene difluoride (PVDF) membrane in 0.
Protein samples were heated at 100[degrees]C for 5 min, separated on 8% SDS-PAGE, and transferred to polyvinylidene difluoride (PVDF) membrane (Amersham, Piscataway, NJ).
Subsequently, we subjected the r-survivin sample to 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by transfer to a polyvinylidene difluoride membrane.
Recombinant livin protein was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride membrane (Millipore).
Sera were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with or without 2-mercaptoethanol (2-ME), and electrophoretically transferred to polyvinylidene difluoride (PVDF) membranes (12).
Recombinant survivin protein was separated by 100 mL/L sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride membrane (Milipore).
The proteins were transferred to ProBlott polyvinylidene difluoride (PVDF) membranes (PE Biosystems) at 2 mA/[cm.
Samples were electrophoresed on 4-20% sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) minigels (31) with subsequent electrotransfer to PSQ-grade polyvinylidene difluoride membranes (Sigma), using a three-buffer semidry blotting system (32)(ESA); the stained bands were Edman sequenced (31).