Polycations have gained increasing attention as nonviral gene delivery vectors in the past decades.
Although gene transfection of plasmid and/or ON complexed with anionic liposomes through polycations is documented, (6,7,9) little attention seems to be paid to understanding their physicochemical characteristics and cellular uptake mechanisms.
Finally, once the complex enters the nucleus, ON may have to dissociate from the polycations in order to be read by the host transcription machinery.
Transfection complex formation is based on the interaction of the positively charged polycations with the negatively charged phosphate groups of the nucleic acid.
Transferrinpolycation-DNA complexes: the effect of polycations on the structure of the complex and DNA delivery to calls.
The addition of Elan's targeting technologies to delivery vehicles such as Lipid Polycation DNA may provide targeted, systemic approaches for the delivery of therapeutic genes.
The promising clinical and pre-clinical data that we are generating in studies of both our DCC and Lipid Polycation DNA delivery platforms validate our plan to develop multiple gene delivery strategies," said H.
In one of these systems, designated "LPD" for lipid-polycation-DNA, the DNA is condensed through the addition of a polycation to form small, degradation resistant cores encapsulated in a lipid shell.
2+], other di- and trivalent cations, and organic polycations
that poorly enter inside the cell, rapidly increased [Ca.
In this case, the process taking place was elec-trospraying and can be explained by the repulsive interactions between the polycations
along the chitosan chains, which consequently did not reach sufficient chain entanglements for fiber formation.
24] by the fact that a bilayer of PEO adsorbed on MMT layers acted as inhibitor for each further adsorption of polycations
Since the PANI chains are polycations
, the resulting attractive electrostatic interactions may play a role in the polymerization coating process by PANI of the mica particles.