To make an abzyme that specifically cleaves phenylacetate
, the researchers obtained antibodies that bind to another longer-lived molecule that is similar in form to phenylacetate
's fleeting transition-state.
rate of phenylacetate
hydrolysis) and serum PON1 paraoxonase (PXN) activity (i.
The colonies were negative for nitrate reduction to nitrite, oxidase, indole production, gelatin hydrolysis, N-acetyl-D-glucosamine; and assimilation of arabinose, mannose, maltose, caprate, adipate, citrate, and phenylacetate
was used as the substrate in these assays, and hydrolysis rates were reported in units per milliliter.
A further modification of the salt-stimulation technique combined the salt-stimulation technique with the differential inhibition of the phenylacetate
(PA) hydrolysis by 0.
We measured three PON1 enzyme activities in plasma from mothers and children, using paraoxonase (POase), chlorpyrifos oxonase (CPOase), and phenylacetate
arylesterase (AREase) according to published protocols (Jarvik et al.
To prevent an ammonia spike during Yang's labor on November 17, 2009, Enns and his colleagues put part one of their plan in motion, first infusing a solution of sodium phenylacetate
and sodium benzoate, along with arginine hydrochloride, into Yang's bloodstream to safely capture the toxin.
Moreover, we measured the activity of PON1 with paraoxon as substrate, whereas the previous study (5) used phenylacetate
as a substrate.
PON1 activity was assessed by measuring the hydrolysis of phenylacetate
(added to plasma) to acetic acid and phenol, as previously described (Chen et al.
In addition, different forms of the enzyme have different substrate specificities toward organophosphates, which they do not exhibit toward phenylacetate
The level of PON 1 activity in each blood sample was determined by an assay that measures hydrolysis of phenylacetate
PON1 activity was determined by use of phenylacetate
as substrate (11).