phase-contrast microscope


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Related to phase-contrast microscope: Interference microscope

microscope

 [mi´kro-skōp]
an instrument used to obtain an enlarged image of small objects and reveal details of structure not otherwise distinguishable.
The light path of a darkfield microscope. From Hart and Shears, 1997.
acoustic microscope one using very high frequency ultrasound waves, which are focused on the object; the reflected beam is converted to an image by electronic processing.
binocular microscope one with two eyepieces, permitting use of both eyes simultaneously.
compound microscope one consisting of two lens systems whereby the image formed by the system near the object is magnified by the one nearer the eye.
darkfield microscope one so constructed that illumination is from the side of the field so that details appear light against a dark background.
electron microscope one in which an electron beam, instead of light, forms an image for viewing, allowing much greater magnification and resolution. The image may be viewed on a fluorescent screen or may be photographed. Types include scanning and transmission electron microscopes.
fluorescence microscope one used for the examination of specimens stained with fluorochromes or fluorochrome complexes, e.g., a fluorescein-labeled antibody, which fluoresces in ultraviolet light.
light microscope one in which the specimen is viewed under ordinary illumination.
operating microscope one designed for use in performance of delicate surgical procedures, e.g., on the middle ear or small vessels of the heart.
phase microscope (phase-contrast microscope) a microscope that alters the phase relationships of the light passing through and that passing around the object, the contrast permitting visualization of the object without the necessity for staining or other special preparation.
scanning electron microscope (SEM) an electron microscope that produces a high magnification image of the surface of a metal-coated specimen by scanning an electron beam and building an image from the electrons reflected at each point.
simple microscope one that consists of a single lens.
slit lamp microscope a corneal microscope with a special attachment that permits examination of the endothelium on the posterior surface of the cornea.
stereoscopic microscope a binocular microscope modified to give a three-dimensional view of the specimen.
transmission electron microscope (TEM) an electron microscope that produces highly magnified images of ultrathin tissue sections or other specimens. An electron beam passes through the metal-impregnated specimen and is focused by magnetic lenses into an image.
x-ray microscope one in which x-rays are used instead of light, the image usually being reproduced on film.

phase mi·cro·scope

, phase-contrast microscope
a specially constructed microscope that has a special condenser and objective containing a phase-shifting ring whereby small differences in index of refraction are made visible as intensity or contrast differences in the image; particularly useful for examining structural details in transparent specimens such as living or unstained cells and tissues.

phase mi·cro·scope

, phase-contrast microscope (fāz mī'krŏ-skōp, fāz-kon'trast)
A specially constructed microscope that has a special condenser and objective containing a phase-shifting ring whereby small differences in index of refraction are made visible as intensity or contrast differences in the image; particularly useful for examining structural details in transparent specimens such as living or unstained cells and tissues.
References in periodicals archive ?
If odors of ethyl acetate (airplane glue/ nail polish remover), amyl acetate (banana skin), or other off-odors appear in juice or must, examine immediately under a phase-contrast microscope for yeasts that are not the round/ovoid shape of Saccharomyces.
Percentage motility was observed under phase-contrast microscope (40 A-).
Two hundred spermatozoa were counted under a phase-contrast microscope (100 A-) for acrosomal integrity which was indexed as percentage of normal ruffled swollen or absent acrosomes (Ijaz et al.
One drop of the incubated semen was placed on a pre-warmed glass slide and examined under phase-contrast microscope (40A-).
Fluorescent phase-contrast microscope for live cell analysis and for rapid fluorescence microscopy images.
Observation of small drop of incubated mixture was carried out under a phase-contrast microscope (X400) and two hundred spermatozoa were counted for swelling/coiling of tail (Adeel et al.
Sperm acrosomal integrity was determined by mixing 500 ul of semen samples with 50ul of 1% formal citrate solution and a drop of semen sample was examined under phase-contrast microscope (1000X).

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