periodic acid-Schiff stain

(redirected from Periodic acid Schiff)

per·i·od·ic ac·id-·Schiff stain (PAS),

(shif),
a tissue-staining procedure in which 1,2-glycol groupings are first oxidized with periodic acid to aldehydes that then react with the sulfite leucofuchsin reagent of Schiff and turn red-violet; strong staining occurs with polysaccharides, such as glycogen, and mucopolysaccharides of epithelial mucins, basement membranes, and connective tissue.
Synonym(s): PAS stain

Schiff,

Hugo, German chemist in Florence, 1834-1915.
Kasten fluorescent Schiff reagents - see under Kasten
ninhydrin-Schiff stain for proteins
periodic acid-Schiff stain - a tissue-staining procedure. Synonym(s): PAS stain
Schiff base - condensation products of aldehydes and ketones with primary amine. Synonym(s): aldimine
Schiff reagent - used for aldehydes and in histochemistry to detect polysaccharides, DNA, and proteins.

per·i·od·ic ac·id-Schiff stain

(PAS) (pērē-odik asid shif stān)
A clinical tissue-staining procedure.
References in periodicals archive ?
Several studies have suggested that Periodic acid Schiff staining of nail clippings may be a very sensitive method for diagnosis of onychomycosis7,10,11.
Pulmonary alveolar proteinosis (PAP) is a rare lung disease where periodic acid Schiff (PAS)-positive eosinophilic material accumulates in the alveoli of the lungs.
Gomori methenamine silver (GMS) and periodic acid schiff stain (PAS) stains were performed and highlighted thin filamentous organisms.
The specimens are rehydrated in 70% ethyl alcohol and then placed successively in periodic acid Schiff reagent, sodium metasulfite, Gill's haematoxylin, and Scott's tap water substitute for 2 minutes each, rinsing in two changes of tap water in between each step.
Staining a smear of aspirated material with mucus specific stain periodic acid Schiff (PAS) confirmed saliva.
Regions Techniques Proximal Medial Distal Intestine Intestine Intestine Periodic Acid Schiff (+) to (+ +) (++) to (+++) (PAS) (+++) Alcian Blue (AB) (+ +) to (+ + +) (++) to (+++) pH 1.
The renal tissues slides were stained with periodic acid Schiff (PAS) reagent and examined light microscopy.
Cytochemically, these cells were negative for myeloperoxidase, Sudan black, acid phosphatase and periodic acid Schiff, while few cells showed granular and dot positivity with non-specific esterase as described in an older literature [5].
Tissue samples were processed routinely, sectioned, mounted on glass slides, and stained with hematoxylin and eosin or periodic acid Schiff (PAS) for light microscopic examination.
And if" you do a biopsy, always [have the pathologist do] a periodic acid Schiff stain on the specimen," Dr.

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