Other Pasteurellaceae, particularly Bibersteinia trehalosi and Pasteurella multocida, have been more frequently isolated from pneumonia-affected animals during natural outbreaks than has M.
Pasteurellaceae were isolated and identified by using routine methods (25) and then stored at -80[degrees]C in 30% buffered glycerol in brain-heart infusion agar (Hardy Diagnostics).
Pulsed-Field Gel Electrophoresis for Pasteurellaceae
When available, Pasteurellaceae isolated from the study animals were obtained from the Washington Animal Disease Diagnostic Laboratory (Pullman, WA, USA).
In contrast, the PFGE strain types of Pasteurellaceae isolated from within single outbreaks ranged from 0 to 7, including 0-7 B.
ovipneumoniae (38) or by leukotoxin-expressing Pasteurellaceae (39), albeit by different mechanisms.
The NucleoLink surface was applied previously to the detection of the bovine leukemia virus (11), Fusarium graminearum (21), and the Pasteurellaceae
bacterial family (22) by taking advantage of the covalent linkage formed by a previously published carbodumide condensation reaction (23) between the 5'-phosphorylated oligonucleotide and a reactive group on the surface.