A review of the literature offers no conclusive links of changing plasma PAI1 levels to initiation of parturition, though it is involved in proteolytic events in the decidua.
Prognostic value of PAI1 in invasive breast cancer: evidence that tumor-specific factors are more important than genetic variation in regulating PAI1 expression," Cancer Epidemiology Biomarkers and Prevention, vol.
PCR with allele-specific oligonucleotide hybridization (11, 14, 25), amplification with sequence-specific primers (26-29), ASRA (18, 30), and single-strand conformation polymorphism (31) have been used to genotype the PAI1 promoter polymorphism.
In the present study, we used probe hybridization and FRET to monitor product accumulation during rapid-cycle DNA amplification and to determine PAI1 promoter genotypes by melting-curve analysis of the amplified fragment on the LightCycler.
Furthermore, because hands-on time is short, this method permits PAI1 genotyping in a very economical manner.