Northern blotting


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A technique used to detect a specific mRNA sequence, which consists of electrophoresing RNA fragments, transferring those fragments to a membrane, then adding a labelled nucleic acid probe to the milieu

Northern blotting

Molecular biology A technique used to detect the presence of a specific mRNA sequence. See Blotting, Hybridization, Probe, RNA, Southern blotting.

Northern transfer

or

Northern blotting

a method for transferring BANDS of RNA, separated by GEL ELECTROPHORESIS, from the electrophoresis gel to a membrane support. The method is named by analogy with SOUTHERN TRANSFER.
References in periodicals archive ?
In preliminary control experiments, when Northern blotting was carried out with individual probes alone, using identical hybridization and wash conditions, the hybridization patterns showed single bands of appropriate sizes for each probe (18S rRNA, 2000 nt; FAMTase mRNA, 1250 nt).
To determine the tissue distribution and developmental expression of the putative FAMTase, Northern blotting was carried out using the 450-bp clone as a probe.
This retains the advantages of RPA over northern blotting, which is its greater sample capacity and hybridization efficiency by performing the digestion in solution rather than on the membrane.
Compared with manual PCR quantification techniques, such as Northern blotting or RNase protection assays, real-time PCR offers an enormous time savings, greater sensitivity, superior precision, and a larger dynamic range.
LPL mRNA levels were determined by Northern blotting and laser scanning of autoradiographs.
It is a more sensitive method to quantify gene expression than conventional methods used for mRNA evaluation, such as Northern blotting, dot-blot analysis, and the RNase protection assay.
We isolated the full-length cDNA of porcine FoxO1 gene using RACE, confirmed by visual Northern blotting.
The protein and mRNA contents of UGT2B1 also were not detected in the fetal rat liver, but a developmental increase in newborn rat liver was detected by Western and Northern blotting analysis.
Neither genomic Southern blotting nor Northern blotting of tumor RNA can be performed successfully with the degraded nucleic acids obtained from fixed and embedded tissue specimens.
To estimate the expression sites of the two genes in the foot, RNA was isolated separately from the distal end and from the remainder of the foot and was also analyzed by northern blotting.
We next examined the effects of cadmium on the transcript level of bcl-2 by Northern blotting, comparing with that of c-myc as the immediate early gene.
Although duplicate performance of experiments may seem time consuming, our experience has shown that the extra effort expended at this stage is more than repaid by reducing unnecessary cloning, sequencing, and Northern blotting later in the process.

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