Northern blotting


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A technique used to detect a specific mRNA sequence, which consists of electrophoresing RNA fragments, transferring those fragments to a membrane, then adding a labelled nucleic acid probe to the milieu

Northern blotting

Molecular biology A technique used to detect the presence of a specific mRNA sequence. See Blotting, Hybridization, Probe, RNA, Southern blotting.

Northern transfer

or

Northern blotting

a method for transferring BANDS of RNA, separated by GEL ELECTROPHORESIS, from the electrophoresis gel to a membrane support. The method is named by analogy with SOUTHERN TRANSFER.
References in periodicals archive ?
The purified RNA can be used in a number of downstream applications including real time PCR, RT-PCR, Northern blotting, RNase protection, primer extension, expression array assays and NGS.
Among the topics are detecting changes in global genome methylation using the cytosine-extension assay, isoschizomers and amplified fragment length polymorphism for detecting specific cytosine methylation changes, northern blotting techniques for small RNAs, cloning new small RNA sequences, cDNA libraries for virus-induced gene silencing, and detecting and quantifying DNA strand breaks using the random oligonucleotide primed synthesis (ROPS) assay.
The recombinant DNA was labeled with a digoxigenin RNA labeling kit (Roche) to prepare sense and antisense cRNA probes for Northern blotting and in situ hybridization.
The expression level of each gene in both the yeast and mold morphotypes of four Hc strains was examined by northern blotting and realtime PCR.
The PRSS11-L mRNA is widely expressed in several tissues throughout the body by multi-tissue Northern blotting.
2]-As quantified in these latter studies using reverse Northern blotting, changes in several hypothalamic mRNAs induced by waterborne environmentally relevant levels of octylphenol in these studies were approximately 2-fold.
Because Northern blotting detected no wild-type LDHA mRNA, the occurrence of some mutations in the LDHA gene was suspected.
When checked by a number of methods including quantitative RT-PCR (6, 35), Northern blotting (33, 34, 36), and protein expression (33, 34), most differentially expressed genes have been confirmed.
This retains the advantages of RPA over northern blotting, which is its greater sample capacity and hybridization efficiency by performing the digestion in solution rather than on the membrane.
Compared with manual PCR quantification techniques, such as Northern blotting or RNase protection assays, real-time PCR offers an enormous time savings, greater sensitivity, superior precision, and a larger dynamic range.

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