Northern blot analysis


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North·ern blot a·nal·y·sis

a procedure similar to the Southern blot analysis, used to separate and identify RNA fragments; typically through transferring (blotting) RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]

Northern blot analysis

n.
A technique for identifying specific sequences of RNA in which RNA molecules are separated by electrophoresis, transferred to nitrocellulose, and identified with a suitable probe.

North·ern blot a·nal·y·sis

(nōr'dhĕrn blot ă-nal'i-sis)
A procedure similar to the Southern blot analysis, used mostly to separate and identify RNA fragments; typically done through transferring RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]
References in periodicals archive ?
Only a select number of genes were found to be up-regulated by HDI using microarray analysis, and these effects were confirmed using Northern blot analysis.
Total RNA samples from hepatoma cell lines containing various amounts of 2-5AS mRNA were analyzed by C-PCR and Northern blot analysis, and the results were compared (Fig.
Linear and reproducible quantification was confirmed by the reconstitution experiment, correlating the results with those obtained by Northern blot analysis.
As we demonstrated previously by Northern blot analysis (4) and semiquantitative RT-PCR (28), expression of human ABCA1 mRNA in macrophages is regulated in a differentiation-dependent fashion.
To determine if Roundup reduced StAR protein levels by decreasing StAR mRNA levels, we performed Northern blot analysis (Figure 7).
Molecular biology experience including isolation of RNA, DNA, and proteins, Western and Northern blot analysis, RTPCR analysis of mRNAs, and immunohistochemistry are desirable.
RNA purification is a critical first step for preparing biological samples for a multitude of analytical techniques including real-time quantitative PCR, reverse transcription PCR, microarrays, Northern blot analysis, nuclease protection assays, RNA mapping, in vitro translation and DNA library construction.

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