Northern blot analysis


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North·ern blot a·nal·y·sis

a procedure similar to the Southern blot analysis, used to separate and identify RNA fragments; typically through transferring (blotting) RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]

Northern blot analysis

n.
A technique for identifying specific sequences of RNA in which RNA molecules are separated by electrophoresis, transferred to nitrocellulose, and identified with a suitable probe.

North·ern blot a·nal·y·sis

(nōr'dhĕrn blot ă-nal'i-sis)
A procedure similar to the Southern blot analysis, used mostly to separate and identify RNA fragments; typically done through transferring RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]
References in periodicals archive ?
Northern blot analysis of the p53 gene showed that, in PAXgene tubes, the mRNA was preserved for 7 days at room temperature, although the intensity of the transcript band diminished slightly at 5 and 7 days of storage.
Only a select number of genes were found to be up-regulated by HDI using microarray analysis, and these effects were confirmed using Northern blot analysis.
The Northern blot analysis demonstrated the occurrence of a 1.
Total RNA samples from hepatoma cell lines containing various amounts of 2-5AS mRNA were analyzed by C-PCR and Northern blot analysis, and the results were compared (Fig.
6 shows the Northern blot analysis of ABCA1 mRNA in macrophages from three healthy donors (Fig.
To determine if Roundup reduced StAR protein levels by decreasing StAR mRNA levels, we performed Northern blot analysis (Figure 7).
Molecular biology experience including isolation of RNA, DNA, and proteins, Western and Northern blot analysis, RTPCR analysis of mRNAs, and immunohistochemistry are desirable.

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