Following a 30 second incubation, 150 [micro]L of ten percent nigrosin
solution is added, and mixed.
1 [micro]L stained with Ponceau S or nigrosin were adequate for quantitative densitometry or elution.
Ponceau S and nigrosin protein staining techniques were presented.
Fresh ejaculates were evaluated for volume, motility (diluted with normal saline), progressive motility, live spermatozoa (eosin nigrosin
stain), sperm concentration or density (sperm cells were counted in four squares of a hemocytometer after 1:200 dilution of semen with 0.
The eosin nigrosin
exclusion and the hyposmotic swelling test were combined to a form a single test (HOS-EN test) to identify four types of spermatozoa according to membrane integrity and function.
A The addition of India ink or nigrosin
to CSF sediment and subsequent examination by light microscopy has been commonly used to detect encapsulated strains of Cryptococcus neoformans.
Spermatozoa viability: A thin smear of thawed semen and Eosin and Nigrosin
as described by Zemjanis (1970) was prepared on a microscopic slide and evaluated it under a phase contrast microscope (X1000).
One drop of semen was placed on a glass slide and mixed with a drop of iso molar supravital stain [eosin (1 % w/v) nigrosin
(5 % w/v) in 3 % tri-sodium citrate dihydrate solution] to prepare a uniform air dried thin smear and was observed under phase-contrast microscope (100 A-).
Spermatozoa viability: A 50 u l drop of the thawed semen was placed on a pre-warmed (37degC) glass slide and mixed with 50 u l of Eosin and Nigrosin
(Zemjanis, 1970) using an applicator stick.