nested polymerase chain reaction


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nested polymerase chain reaction

use of the PCR in series such that a specified piece of DNA is amplified and then a portion contained within the first piece is amplified further; used where extremely low amounts of DNA are present, or where there are problems with background or contaminating DNA.
References in periodicals archive ?
Babesiae were detected in cervids by using specific nested polymerase chain reaction (PCR) that allowed discrimination between B.
Molecular diagnostic assays, such as nested polymerase chain reaction and a species-specific DNA probe from the ribosomal DNA complex, have been useful to identify P.
We describe a nested polymerase chain reaction for the identification of Borrelia species from serum of patients with unidentified fevers.
Dogs and their owners were sampled as pairs, by fungal culture and nested polymerase chain reaction (PCR).
Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction.
The tubes from the remaining 47 samples were subjected to DNA analysis by a nested polymerase chain reaction (PCR) (8).
Complementary DNA was amplified by nested polymerase chain reaction (PCR) by using primers from the small (S) RNA segment of C-CHFV (4).
A section of the SSU (18S) rRNA gene was amplified by nested polymerase chain reaction (PCR) as described (14), using the forward primers 5'-TTCTAGAGCTAATACATGCG-3' and the reverse primer 5'-CCCTAATCCTTCGAAACAGGA-3' for primary PCR.