nested polymerase chain reaction


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nested polymerase chain reaction

use of the PCR in series such that a specified piece of DNA is amplified and then a portion contained within the first piece is amplified further; used where extremely low amounts of DNA are present, or where there are problems with background or contaminating DNA.
References in periodicals archive ?
Early diagnosis and species differentiation in multi drug resistant-tuberculosis by nested polymerase chain reaction targeting hupB gene.
Screening for Crvptosporidium species by using microscopic examination of fecal samples and stained smears, histopathology, and nested polymerase chain reaction for actin gene and 18S ribosomal RNA gene after sequencing of amplified fragments allowed for the identification of C galli.
Serotype specific amplification of Rickettsia tsutsugamushi DNA by nested polymerase chain reaction.
Detection of Borrelia duttonii, a tick-borne relapsing fever agent in central Tanzania, within ticks by flagellin gene-based nested polymerase chain reaction.
1), which describes the molecular detection of Rickettsia typhi and 4 spotted fever group rickettsiae by nested polymerase chain reaction (PCR) in the serum of febrile Korean patients.
Nested polymerase chain reaction for high-sensitivity detection of enteroviral RNA in biological samples.
Babesiae were detected in cervids by using specific nested polymerase chain reaction (PCR) that allowed discrimination between B.
Molecular diagnostic assays, such as nested polymerase chain reaction and a species-specific DNA probe from the ribosomal DNA complex, have been useful to identify P.
We describe a nested polymerase chain reaction for the identification of Borrelia species from serum of patients with unidentified fevers.
Dogs and their owners were sampled as pairs, by fungal culture and nested polymerase chain reaction (PCR).