multiple cloning site


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multiple cloning site (MCS) or polylinker

a synthetic DNA fragment containing restriction sites for a number of RESTRICTION ENZYMES, which is incorporated into a VECTOR to provide multiple cloning sites. By cleaving the MCS with the relevant restriction enzyme at a particular site, insert DNA with compatible COHESIVE ENDS can be inserted into the vector at that site for cloning. see GENE CLONING.
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gene as a selectable marker in order to identify transgenic Drosophila that receive a stable recombinant promoter::lacZ construct via P-element mediated transformation as well as multiple cloning sites upstream of a truncated hs43 promoter and a [beta] galactosidase gene.

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