aeruginosa were processed for the determination of sensitivity against antibiotics, piperacillin, piperacillin + tazobactam, ticarcillin + tazobactam, ceftazidime, cefepime, aztreonam, imipenem, meropenem, gentamicin, amikacin, ciprofloxacin, levofloxacin, on Mueller-Hinton agar
The bacterial inoculums were uniformly swabbed on prepared Mueller-Hinton agar
plates followed by a session of drying, after which three wells of 7 mm diameter each were dug in the agar gel 33 mm apart from one another using a sterile cork borer.
Using a sterile inoculation loop, scrape spores from an agar plate and spread evenly on the surface of a Mueller-Hinton agar
Petri plates containing 20 ml of Mueller-Hinton agar
media were inoculated with 200 [micro]l of diluted cultures by the spread plate technique and were allowed to dry in a sterile chamber.
Using a sterile cotton swab (Hi-Media Lab, Mumbai) the bacterial inoculums were inoculated in Mueller-Hinton agar
Quality control limits for fluconazole disk susceptibility test son Mueller-Hinton agar
with glucose and methylene blue.
Once the causative organism was isolated, it's culture on Mueller-Hinton agar
was subjected to antibiotic sensitivity test using standard antibiotic discs.
The resulting suspension was applied to the surface of over-dried Mueller-Hinton agar
and spread evenly with a sterile cotton-tipped applicator.
10 mL of each bacterial suspension was transferred into the Mueller-Hinton agar
With the use of an inoculating loop, a colony was extracted and was transferred to a Mueller-Hinton agar
Quantification of bacterial growth will be performed through plating 10 [micro]L from each well, in triplicate, on solid Mueller-Hinton agar
followed by 24 hours incubation at 35 [+ or -] 2[degrees]C in normal atmosphere.
plates were overlaid with the inoculum (turbidity equivalent to that of a 0.