Buffers of various types like acetate, phosphate and sulphate in pH range of 2-8 were also studied as mobile phase
2 mL/min mobile phase
flowrate, and 50-50 split aqueous-organic mobile phase
9 [micro]m), P/N 25502-102130 Mobile Phase
A1: 20mM Ammonium formate in water, pH 3, (P/N Ammonium formate A114-50) A2: 20 mM Ammonium formate in water, pH 4 A3: 20 mM Ammonium acetate in water, pH 5, (P/N Ammonium acetate A115-50) A4: 20 mM Ammonium acetate in water, pH 5.
The pore diameter was one of the most important variables, because regulates the pressure and flow that should be applied in the mobile phase
of the column.
For example, bacteria in the mobile phase
will lead to an increase in background absorbance and reduce the quality of the data you generate.
Double mobile phase
TLC was done with 2 ^L of each sample.
The following RP- HPLC conditions were used; wave length 254 nm; mobile phase
flow rate 1 mL/min; sample injection volume 20 L.
The mobile phase
temperature plays a key role for improving the peak shapes of proteins.
The selected mobile phase
with high Rf value was verified into seven ratios (5:1:2, 1:5:2, 1:2:5, 2:1:5, 3:3:3, 5:2:1).
Nine structurally diverse, strongly basic drug compounds were resolved in less than 2-minutes using a mobile phase
Daily working standard solutions of mixture were prepared by suitable dilution of the stock solution with the mobile phase
With the Method Toolbox, chemists can analyze up to 96 methods, 16 mobile phase
solvents and six columns all in one batch.