miniprep


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miniprep

A rapid, abbreviated method for identifying cloned DNA in a culture of host bacteria.

Steps in minipreps include lysis of host cells, cutting the cloned DNA/vector with a restriction endonuclease, and running the digest on an electrophoretic gel to identify characteristic bands.
References in periodicals archive ?
By using Bertin's Precellys Evolution Homogenizer together with Zymo Research's ZymoBIOMICS Microbial Community Standards and ZymoBIOMICS DNA Miniprep Kit for purification of the DNA, researchers are afforded unbiased cell lysis in only one minute.
Plasmid DNA was purified using QIAprep spin miniprep kit (QIAgen, Hilden, Germany), linearized and quantified with the Nanodrop ND-1000 instrument (Wilmington, DE, USA) following transcription to RNA with T7 RNA polymerase (Promega, Madison, WI, USA).
Blood samples from all patients were collected and subjected to RNA extraction by RNA Miniprep Super Kit (Bio Basic Inc, Canada).
Genomic DNA was extracted from pure cultures using HiPurA[TM] Bacteria Genomic DNA Miniprep Purification Spin Kit (Hi-Media, India) according to the manufacturer's instructions.
A Gene JET Plasmid Miniprep Kit (Fermentas, Vilnius, Lithuania) was used to purify putative full-length clones followed by sequencing the cloned insert both orientations (Macrogen Inc.
The plasmid was extracted from positive clone using GeneJET(tm) Plasmid Miniprep Kit (Thermoscientific, Lithuania) for further use as positive control and evaluation of sensitivity of RT-PCR assay.
The buccal cells donated by the subjects were collected in Resuspension Solution (GenElute Mammalian Genomic DNA Miniprep Kit, Sigma, Germany) with the use of sterile foam-tipped applicators (Puritan, USA).
Single plaques were harvested and amplified on Vero cells before RNA isolation by using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich, Zwijndrecht, the Netherlands) according to the manufacturer's instructions.
All vancomycin-resistant isolates were screened for the presence of the vancomycin resistance genes vanA, B and Cl, 2, 3 with DNA purified using the ZR Fungal/Bacterial DNA MiniPrep kit (Zymo Research, USA) according to the manufacturer's instructions.
La extraccion de ADN genomico se realizo utilizando el estuche comercial AxyPrep Blood Genomic DNA Miniprep Kit (Axygen Biosciences, USA), siguiendo las instrucciones del fabricante.