confocal microscopy

(redirected from Microscopy, confocal)

confocal microscopy

Microscopy that permits high-resolution analysis of serial optical sections (microscopic tomograms) into the depths of tissues or cells.
See also: microscopy


examination with a microscope.

confocal microscopy
a technique for obtaining high resolution images and 3-D reconstructions of biological specimens; a laser light beam is expanded to make optimal use of the optics in the objective lens and is turned into a scanning beam via an x-y deflection mechanism and is focused to a small spot by the objective lens onto a fluorescent specimen. The mixture of reflected light and emitted fluorescent light is captured by the same objective and after conversion into a static beam by the x-y scanner device is focused onto a photodetector (photomultiplier) via a dichroic mirror (beam splitter) to create the final image. Called also laser scanning microscopy; confocal scanning laser microscopy.
immunoelectron microscopy
the mixing of antibody with an antigen such as a virus on a specimen grid so as to increase the probability of visualizing a virus and to identify (type) the kind of virus present in the specimen, or antibody may be conjugated with gold and used to visualize and determine the location of specific antigenic determinants on a specimen.
References in periodicals archive ?
Specific techniques are then treated in separate contributions, including holographic microscopy, confocal laser scanning microscopy, optical projection tomography, electron microscopy, magnetic resonance and X-ray imaging, thin light-sheets, and ex-situ macro photography, with each chapter giving an introduction and discussing technical specifications, operator considerations, ideal environments, and tradeoffs.
Research in the section of pathobiology is focused primarily on the application of morphological techniques - scanning and transmission electron microscopy, confocal and fluorescence microscopy, immunohistochemistry and autoradiography - in the study of cell cultures, the pathophysiology of diseases in the GI tract, muscle, the lungs, the eye, and the inner ear.
7-AAD/DNA complexes can be excited by argon-ion laser and emit fluorescence with a maxima of 647 nm, making this nucleic acid stain useful for multicolor fluorescence microscopy, confocal laser-scanning microscopy and flow cytometry.
As one of the most important advances achieved in optical microscopy, confocal laser scanning microscopy (CLSM) offers several advantages over conventional fluorescence microscopy, including control of depth of field, elimination of image-degrading out-of-focus information, and collection of serial optical sections from thick specimens.
Unlike standard microscopy, confocal microscopy can strongly suppress out-of-focus light.
It is one of the market leaders in each of its business areas: Microscopy, Confocal Laser Scanning Microscopy with corresponding Imaging Systems, Specimen Preparation, and Medical Equipment.
After fluorescent microspheres were injected into the blood, samples of the digestive gland were excised, arterioles were prepared for microscopy, confocal laser scanning micrographs were collected and microspheres and cells were counted.
One of two major forms of laser scanning microscopy, confocal laser scanning microscopy uses a beam of laser light focused into a small point of a specimen and can be moved with a computer-controlled scanning mirror.
Investigators did so by using computer simulations of particle gelation; studying product structure using light microscopy, confocal laser scanning microscopy, electron microscopy and dynamic light scattering of gels and emulsions; examining small-deformation rheology and large-deformation rheology of gels and emulsions; and using surface chemical and physical methods on dairy emulsions.
It is one of the market leaders in each of the fields Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment.
This technology can be applied to images of different magnifications, resolution, and modalities, including electron microscopy, optical microscopy, confocal microscopy, radiology, magnetic resonance imaging (MRI), conventional photography, and other sophisticated imaging variants.